Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

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Ostatnie publikacje
1.  Nakielski P., Kosik-Kozioł A., Rinoldi C., Rybak D., Namdev M., Jacob W., Lehmann T., Głowacki M., Bogusz S., Rzepna M., Marinelli M., Lanzi M., Dror S., Sarah M., Dmitriy S., Pierini F., Injectable PLGA Microscaffolds with Laser-Induced Enhanced Microporosity for Nucleus Pulposus Cell Delivery, Small, ISSN: 1613-6810, DOI: 10.1002/smll.202404963, pp.2404963-1-15, 2024

Streszczenie:
Intervertebral disc (IVD) degeneration is a leading cause of lower back pain (LBP). Current treatments primarily address symptoms without halting the degenerative process. Cell transplantation offers a promising approach for early-stage IVD degeneration, but challenges such as cell viability, retention, and harsh host environments limit its efficacy. This study aimed to compare the injectability and biocompatibility of human nucleus pulposus cells (hNPC) attached to two types of microscaffolds designed for minimally invasive delivery to IVD. Microscaffolds are developed from poly(lactic-co-glycolic acid) (PLGA) using electrospinning and femtosecond laser structuration. These microscaffolds are tested for their physical properties, injectability, and biocompatibility. This study evaluates cell adhesion, proliferation, and survival in vitro and ex vivo within a hydrogel-based nucleus pulposus model. The microscaffolds demonstrate enhanced surface architecture, facilitating cell adhesion and proliferation. Laser structuration improved porosity, supporting cell attachment and extracellular matrix deposition. Injectability tests show that microscaffolds can be delivered through small-gauge needles with minimal force, maintaining high cell viability. The findings suggest that laser-structured PLGA microscaffolds are viable for minimally invasive cell delivery. These microscaffolds enhance cell viability and retention, offering potential improvements in the therapeutic efficiency of cell-based treatments for discogenic LBP.

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Rinoldi C. - IPPT PAN
Rybak D. - IPPT PAN
Namdev M. - inna afiliacja
Jacob W. - inna afiliacja
Lehmann T. - inna afiliacja
Głowacki M. - Jagiellonian University (PL)
Bogusz S. - inna afiliacja
Rzepna M. - inna afiliacja
Marinelli M. - inna afiliacja
Lanzi M. - University of Bologna (IT)
Dror S. - inna afiliacja
Sarah M. - inna afiliacja
Dmitriy S. - inna afiliacja
Pierini F. - IPPT PAN
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