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Streszczenie:
Engineering tissue-like scaffolds that can mimic the microstructure, architecture, topology, and mechanical properties of native tissues while offering an excellent environment for cellular growth has remained an unmet need. To address these challenges, multi-compartment composite fibers are fabricated. These fibers can be assembled through textile processes to tailor tissue-level mechanical and electrical properties independent of cellular level components. Textile technologies also allow controlling the distribution of different cell types and microstructure of fabricated constructs and directing cellular growth within 3D microenvironment. Here, we engineered composite fibers from biocompatible cores and biologically relevant hydrogel sheaths. The fibers are mechanically robust to be assembled using textile processes and could support adhesion, proliferation and maturation of cell populations important for engineering of skeletal muscles. We also demonstrated that the changes in the electrical conductivity of the multi-compartment fibers could significantly enhance myogenesis in vitro.

Słowa kluczowe:
reinforced fibers, biotextiles, tissue engineering, organ weaving, interpenetrating network hydrogels, skeletal muscles

Streszczenie:
Tendon injuries are frequent and occur in the elderly, young, and athletic populations. The inadequate number of donors combined with many challenges associated with autografts, allografts, xenografts, and prosthetic devices have added to the value of engineering biological substitutes, which can be implanted to repair the damaged tendons. Electrospun scaffolds have the potential to mimic the native tissue structure along with desired mechanical properties and, thus, have attracted noticeable attention. In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel. The whole construct composition was optimized to achieve adequate mechanical and physical properties as well as cell viability and proliferation. Mesenchymal stem cells (MSCs) were differentiated by the addition of bone morphogenetic protein 12 (BMP-12). To mimic the natural function of tendons, the cell-laden scaffolds were mechanically stimulated using a custom-built bioreactor. The synergistic effect of mechanical and biochemical stimulation was observed in terms of enhanced cell viability, proliferation, alignment, and tenogenic differentiation. The results suggested that the proposed constructs can be used for engineering functional tendons.

Słowa kluczowe:
tendon tissue engineering, composite scaffolds, nanofibrous materials, mechanical stimulation, stem cell differentiation

Streszczenie:
Residential attics has the potential to be one of the most energy efficient building components by combining thermal processes of attic floor insulation, attic air space, ventilation in attics, and solar collecting roof decks. Large amounts of solar energy collected by the roofs in cooling-dominated and mixed climates generate excess cooling loads, which need to be removed from the building by the space conditioning systems. This paper investigates potential ways to improve the thermal design of the residential home attics to minimize the cooling energy consumption in the cooling-dominated and mixed climates. Dynamic thermal characteristics of thick attic floor insulations and blends of phase change materials (PCMs) with insulations are analyzed. Both approaches can provide notable reductions of thermal loads at the attic level. In addition, a significant time shift of peak-hour loads can move a major operation time for air conditioning system from the daytime peak hours to nighttime low demand hours. A reverse heat flow direction can be observed during the day in the case of really thick layers of bulk insulation or PCM-enhanced insulations, compared to the rest of the building envelope components. This effect may provide free passive cooling to the building, and can be very useful in locations of double electrical tariffs with high daytime peak-hour electric energy rates and less-expensive off-peak energy cost. In both of the above cases, an addition of PCM to the bulk insulation brings substantial performance enhancement not available for traditional insulation applications. This paper presents a short overview of dynamic material characteristics and energy performance data necessary for future dynamic applications of different configurations of the attic floor insulation and PCM-insulation blends in residential homes. A series of whole-building scale and material scale numerical simulations were performed on a single story ranch house to analyze potential energy savings and optimize location of PCM within the attic insulation.

Słowa kluczowe:
Building envelopes, Attics, Thermal mass, Insulation