Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

Partnerzy

Amy McMahon


Ostatnie publikacje
1.  Rabcuka J., Smethurst P. A., Dammert K., Saker J., Aran G., Walsh G. M., Tan J. C. G., Codinach M., McTaggart K., Marks D. C., Bakker S. J. L., McMahon A., Di Angelantonio E., Roberts D. J., Błoński S., Korczyk P.M., Shirakami A., Cardigan R., Swietach P., Assessing the kinetics of oxygen-unloading from red cells using FlowScore, a flow-cytometric proxy of the functional quality of blood, eBioMedicine, ISSN: 2352-3964, DOI: 10.1016/j.ebiom.2024.105498, Vol.111, pp.105498-105498, 2025

Streszczenie:
Background
Metrics evaluating the functional quality of red blood cells (RBCs) must consider their role in oxygen delivery. Whereas oxygen-carrying capacity is routinely reported using haemoglobin assays, the rate of oxygen exchange is not measured, yet also important for tissue oxygenation. Since oxygen-unloading depends on the diffusion pathlength inside RBCs, cell geometry offers a plausible surrogate.
Methods
We related the time-constant of oxygen-unloading (τ), measured using single-cell oxygen saturation imaging, with flow-cytometric variables recorded on a haematology analyser. Experiments compared freshly-drawn RBCs with stored RBCs, wherein metabolic run-down and spherical remodelling hinder oxygen unloading.
Findings
Multivariable regression related τ to a ratio of side- and forward-scatter, referred to herein as FlowScore. FlowScore was able to distinguish, with sensitivity and specificity >80%, freshly drawn blood from blood that underwent storage-related kinetic attrition in O2-handling. Moreover, FlowScore predicted τ restoration upon biochemical rejuvenation of stored blood. Since RBC geometry and metabolic state are related, variants of FlowScore estimated [ATP] and [2,3-diphosphoglycerate]. The veracity of FlowScore was confirmed by four blood-banking systems (Australia, Canada, England, Spain). Applying FlowScore to data from the COMPARE study revealed a positive association with the time-delay from sample collection to measurement, which was verified experimentally. The LifeLines dataset revealed age, sex, and smoking among factors affecting FlowScore.
Interpretation
We establish FlowScore as a widely-accessible and cost-effective surrogate of RBC oxygen-unloading kinetics. As a metric of a cellular process that is sensitive to storage and disease, we propose FlowScore as an RBC quality marker for blood-banking and haematology.

Słowa kluczowe:
Haematology,Erythrocytes,Storage lesion,Assay,Oxygen transport,Transfusion

Afiliacje autorów:
Rabcuka J. - inna afiliacja
Smethurst P. A. - inna afiliacja
Dammert K. - inna afiliacja
Saker J. - inna afiliacja
Aran G. - inna afiliacja
Walsh G. M. - inna afiliacja
Tan J. C. G. - inna afiliacja
Codinach M. - inna afiliacja
McTaggart K. - inna afiliacja
Marks D. C. - inna afiliacja
Bakker S. J. L. - inna afiliacja
McMahon A. - inna afiliacja
Di Angelantonio E. - inna afiliacja
Roberts D. J. - inna afiliacja
Błoński S. - IPPT PAN
Korczyk P.M. - IPPT PAN
Shirakami A. - inna afiliacja
Cardigan R. - inna afiliacja
Swietach P. - inna afiliacja
140p.

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