1. |
Osial M., Ha G.♦, Vu V.♦, Nguyen P.♦, Nieciecka D.♦, Pietrzyk‑Thel P., Urbanek O., Olusegun S.♦, Wilczewski S.♦, Giersig M., Do H.♦, Dinh T.♦, One-pot synthesis of magnetic hydroxyapatite (SPION/HAp) for 5-fluorouracil delivery and magnetic hyperthermia,
Journal of Nanoparticle Research, ISSN: 1388-0764, DOI: 10.1007/s11051-023-05916-x, Vol.26, No.7, pp.1-23, 2024Streszczenie: This work presents the synthesis and characterization of a composite made of superparamagnetic iron oxide and hydroxyapatite nanoparticles (SPION/HAp) with a well-developed surface for loading anticancer drugs and for use in magnetic hyperthermia and local chemotherapy. The proposed material was obtained by an easy one-pot co-precipitation method with a controlled ratio of SPION to HAp. The morphology was studied by SEM and TEM, indicating rod-like structures for high HAp content in the composite and granule-like structures with increasing SPION. Its crystallinity, elemental composition, and functional groups were determined by X-ray diffraction, EDS, and FT-IR, respectively. The nanocomposite was then stabilized with citrates (CA), polyethylene glycol (PEG), and folic acid (FA) as agents to improve intracellular absorption, while turbidimetric studies confirmed that only citrates effectively stabilized the magnetic carriers to form a colloidal suspension. Subsequently, 5-fluorouracil (5-FU) was loaded into the magnetic carriers and tested in vitro using the L-929 cell line. The studies showed no cytotoxicity of the citrate-stabilized suspension against fibroblasts and some cytotoxicity after 5-FU release. In addition to in vitro studies, the composite was also tested on biomimetic membranes made of DOPC, DOPE, cholesterol, and DOPS lipids using Langmuir trough. The results show that the resulting suspension interacts with biomimetic membranes, while magnetic hyperthermia studies confirm effective heat generation to achieve therapeutic 42–46 °C and improve drug release from magnetic carriers. Słowa kluczowe: SPION, Hydroxyapatite, Magnetic hyperthermia, Drug delivery, 5-fluorouracil, Biomimetic membranes, Nanostructures, Cancer treatment Afiliacje autorów:
Osial M. | - | IPPT PAN | Ha G. | - | inna afiliacja | Vu V. | - | inna afiliacja | Nguyen P. | - | inna afiliacja | Nieciecka D. | - | inna afiliacja | Pietrzyk‑Thel P. | - | IPPT PAN | Urbanek O. | - | IPPT PAN | Olusegun S. | - | inna afiliacja | Wilczewski S. | - | inna afiliacja | Giersig M. | - | IPPT PAN | Do H. | - | inna afiliacja | Dinh T. | - | inna afiliacja |
| | 70p. |
2. |
Nakielski P., Rybak D., Jezierska-Woźniak K.♦, Rinoldi C., Sinderewicz E.♦, Staszkiewicz-Chodor J.♦, Haghighat Bayan M.A., Czelejewska W.♦, Urbanek-Świderska O., Kosik-Kozioł A., Barczewska M.♦, Skomorowski M.♦, Holak P.♦, Lipiński S.♦, Maksymowicz W.♦, Pierini F., Minimally invasive intradiscal delivery of BM-MSCs via fibrous microscaffold carriers,
ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.3c11710, pp.1-16, 2023Streszczenie: Current treatments of degenerated intervertebral discs often provide only temporary relief or address specific causes, necessitating the exploration of alternative therapies. Cell-based regenerative approaches showed promise in many clinical trials, but
limitations such as cell death during injection and a harsh disk environment hinder their effectiveness. Injectable microscaffolds offer a solution by providing a supportive microenvironment for cell delivery and enhancing bioactivity. This study evaluated the
safety and feasibility of electrospun nanofibrous microscaffolds modified with chitosan (CH) and chondroitin sulfate (CS) for treating degenerated NP tissue in a large animal model. The microscaffolds facilitated cell attachment and acted as an effective delivery system, preventing cell leakage under a high disc pressure. Combining microscaffolds with bone marrow-derived mesenchymal stromal cells demonstrated no cytotoxic effects and proliferation over the entire microscaffolds. The administration of cells attached to microscaffolds into the NP positively influenced the regeneration process of the intervertebral disc. Injectable poly(L-lactide-co-glycolide) and poly(L-lactide) microscaffolds enriched with CH or CS, having a fibrous structure, showed the potential to promote intervertebral disc regeneration. These features collectively address critical challenges in the fields of tissue engineering and regenerative medicine, particularly in the context of intervertebral disc degeneration. Słowa kluczowe: microscaffolds,cell carriers,injectable biomaterials,intervertebral disc,laser micromachining,electrospinning Afiliacje autorów:
Nakielski P. | - | IPPT PAN | Rybak D. | - | IPPT PAN | Jezierska-Woźniak K. | - | inna afiliacja | Rinoldi C. | - | IPPT PAN | Sinderewicz E. | - | inna afiliacja | Staszkiewicz-Chodor J. | - | inna afiliacja | Haghighat Bayan M.A. | - | IPPT PAN | Czelejewska W. | - | inna afiliacja | Urbanek-Świderska O. | - | IPPT PAN | Kosik-Kozioł A. | - | IPPT PAN | Barczewska M. | - | University of Warmia and Mazury in Olsztyn (PL) | Skomorowski M. | - | inna afiliacja | Holak P. | - | inna afiliacja | Lipiński S. | - | inna afiliacja | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Pierini F. | - | IPPT PAN |
| | 200p. |
3. |
Urbanek-Świderska O., Moczulska-Heljak M., Wróbel M.♦, Mioduszewski A.♦, Kołbuk-Konieczny D., Advanced Graft Development Approaches for ACL Reconstruction or Regeneration,
Biomedicines, ISSN: 2227-9059, DOI: 10.3390/biomedicines11020507, Vol.11, No.2, pp.507-1-26, 2023Streszczenie: The Anterior Cruciate Ligament (ACL) is one of the major knee ligaments, one which is greatly exposed to injuries. According to the British National Health Society, ACL tears represent around 40% of all knee injuries. The number of ACL injuries has increased rapidly over the past ten years, especially in people from 26–30 years of age. We present a brief background in currently used ACL treatment strategies with a description of surgical reconstruction techniques. According to the well-established method, the PubMed database was then analyzed to scaffold preparation methods and materials. The number of publications and clinical trials over the last almost 30 years were analyzed to determine trends in ACL graft development. Finally, we described selected ACL scaffold development publications of engineering, medical, and business interest. The systematic PubMed database analysis indicated a high interest in collagen for the purpose of ACL graft development, an increased interest in hybrid grafts, a numerical balance in the development of biodegradable and nonbiodegradable grafts, and a low number of clinical trials. The investigation of selected publications indicated that only a few suggest a real possibility of creating healthy tissue. At the same time, many of them focus on specific details and fundamental science. Grafts exhibit a wide range of mechanical properties, mostly because of polymer types and graft morphology. Moreover, most of the research ends at the in vitro stage, using non-certificated polymers, thus requiring a long time before the medical device can be placed on the market. In addition to scientific concerns, official regulations limit the immediate introduction of artificial grafts onto the market. Słowa kluczowe: ligament,biomaterial,tissue engineering,regeneration,implant,scaffold,synthetic polymer,natural polymer Afiliacje autorów:
Urbanek-Świderska O. | - | IPPT PAN | Moczulska-Heljak M. | - | IPPT PAN | Wróbel M. | - | Fraunhofer Institute for Cell Therapy and Immunology IZI (DE) | Mioduszewski A. | - | inna afiliacja | Kołbuk-Konieczny D. | - | IPPT PAN |
| | 100p. |
4. |
Nakielski P., Rinoldi C., Pruchniewski M.♦, Pawłowska S., Gazińska M.♦, Strojny B.♦, Rybak D., Jezierska-Woźniak K.♦, Urbanek O., Denis P., Sinderewicz E.♦, Czelejewska W.♦, Staszkiewicz-Chodor J.♦, Grodzik M.♦, Ziai Y., Barczewska M.♦, Maksymowicz W.♦, Pierini F., Laser-assisted fabrication of injectable nanofibrous cell carriers,
Small, ISSN: 1613-6810, DOI: 10.1002/smll.202104971, Vol.18, No.2, pp.2104971-1-18, 2022Streszczenie: The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications. Afiliacje autorów:
Nakielski P. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Pruchniewski M. | - | inna afiliacja | Pawłowska S. | - | IPPT PAN | Gazińska M. | - | inna afiliacja | Strojny B. | - | inna afiliacja | Rybak D. | - | IPPT PAN | Jezierska-Woźniak K. | - | inna afiliacja | Urbanek O. | - | IPPT PAN | Denis P. | - | IPPT PAN | Sinderewicz E. | - | inna afiliacja | Czelejewska W. | - | inna afiliacja | Staszkiewicz-Chodor J. | - | inna afiliacja | Grodzik M. | - | inna afiliacja | Ziai Y. | - | IPPT PAN | Barczewska M. | - | University of Warmia and Mazury in Olsztyn (PL) | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Pierini F. | - | IPPT PAN |
| | 200p. |
5. |
Kosińska A.♦, Jagielski J.♦, Bieliński D.M.♦, Urbanek O., Wilczopolska M.♦, Frelek-Kozak M.♦, Zaborowska A.♦, Wyszkowska E.♦, Jóźwik I.♦, Structural and chemical changes in He+ bombarded polymers and related performance properties,
JOURNAL OF APPLIED PHYSICS, ISSN: 0021-8979, DOI: 10.1063/5.0099137, Vol.132, pp.074701-1-18, 2022Streszczenie: The paper presents the effect of He+ ion irradiation of selected polymeric materials: poly(tetrafloroethylene), poly(vinyl chloride), ethylene-propylene-diene monomer rubber, nitrile-butadiene rubber, styrene-butadiene rubber, and natural rubber, on their chemical composition, physical structure, and surface topography. The modification was studied by scanning electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and differential scanning calorimetry. Irradiation with a high-energy ion beam leads to the release of significant amounts of hydrogen from the surface layer, resulting in an increase in cross-linking that manifests itself by shrinkage of the surface layer, which in turn causes significant stresses leading to the formation of a crack pattern on the polymer surface. The development of microroughness is combined with oxidation. Shallow range of the ions makes the modified layer “anchored” in the substrate via bulk macromolecules, assuring its good durability and adhesion to elasto-plastic substrates. Changes in the surface layer were manifested by the modification of functional properties of the polymers. The hardness of the layer subjected to the ion irradiation process increases even up to 10 times. After modification with the ion beam, a significant decrease in frictional forces was also observed, even up to 5–6 times. The microscopic analysis of wear traces confirmed that the wear resistance also significantly increased. However, ion bombardment of polymeric materials caused a reduction in their mechanical strength (despite the range limited to the surface layer of the order of micrometers) and electrical resistance, which has a negative impact on the possibility of using the materials in some applications. Afiliacje autorów:
Kosińska A. | - | inna afiliacja | Jagielski J. | - | National Centre for Nuclear Research (PL) | Bieliński D.M. | - | inna afiliacja | Urbanek O. | - | IPPT PAN | Wilczopolska M. | - | inna afiliacja | Frelek-Kozak M. | - | inna afiliacja | Zaborowska A. | - | inna afiliacja | Wyszkowska E. | - | National Centre for Nuclear Research (PL) | Jóźwik I. | - | Institute of Electronic Materials Technology (PL) |
| | 100p. |
6. |
Rinoldi C., Lanzi M.♦, Fiorelli R.♦, Nakielski P., Zembrzycki K., Kowalewski T., Urbanek O., Jezierska-Woźniak K.♦, Maksymowicz W.♦, Camposeo A.♦, Bilewicz R.♦, Pisignano D.♦, Sanai N.♦, Pierini F., Pierini F., Three-dimensional printable conductive semi-interpenetrating polymer network hydrogel for neural tissue applications,
BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.1c00524, Vol.22, No.7, pp.3084-3098, 2021Streszczenie: Intrinsically conducting polymers (ICPs) are widely used to fabricate biomaterials; their application in neural tissue engineering, however, is severely limited because of their hydrophobicity and insufficient mechanical properties. For these reasons, soft conductive polymer hydrogels (CPHs) are recently developed, resulting in a water-based system with tissue-like mechanical, biological, and electrical properties. The strategy of incorporating ICPs as a conductive component into CPHs is recently explored by synthesizing the hydrogel around ICP chains, thus forming a semi-interpenetrating polymer network (semi-IPN). In this work, a novel conductive semi-IPN hydrogel is designed and synthesized. The hybrid hydrogel is based on a poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide) hydrogel where polythiophene is introduced as an ICP to provide the system with good electrical properties. The fabrication of the hybrid hydrogel in an aqueous medium is made possible by modifying and synthesizing the monomers of polythiophene to ensure water solubility. The morphological, chemical, thermal, electrical, electrochemical, and mechanical properties of semi-IPNs were fully investigated. Additionally, the biological response of neural progenitor cells and mesenchymal stem cells in contact with the conductive semi-IPN was evaluated in terms of neural differentiation and proliferation. Lastly, the potential of the hydrogel solution as a 3D printing ink was evaluated through the 3D laser printing method. The presented results revealed that the proposed 3D printable conductive semi-IPN system is a good candidate as a scaffold for neural tissue applications. Afiliacje autorów:
Rinoldi C. | - | IPPT PAN | Lanzi M. | - | University of Bologna (IT) | Fiorelli R. | - | inna afiliacja | Nakielski P. | - | IPPT PAN | Zembrzycki K. | - | IPPT PAN | Kowalewski T. | - | IPPT PAN | Grippo V. | - | inna afiliacja | Urbanek O. | - | IPPT PAN | Jezierska-Woźniak K. | - | inna afiliacja | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Camposeo A. | - | inna afiliacja | Bilewicz R. | - | inna afiliacja | Pisignano D. | - | inna afiliacja | Sanai N. | - | inna afiliacja | Pierini F. | - | IPPT PAN |
| | 140p. |
7. |
Urbanek O., Wysocka A.♦, Nakielski P., Pierini F., Jagielska E.♦, Sabała I.♦, Staphylococcus aureus specific electrospun wound dressings: influence of immobilization technique on antibacterial efficiency of novel enzybiotic,
Pharmaceutics, ISSN: 1999-4923, DOI: 10.3390/pharmaceutics13050711, Vol.13, No.5, pp.711-1-17, 2021Streszczenie: The spread of antimicrobial resistance requires the development of novel strategies to combat superbugs. Bacteriolytic enzymes (enzybiotics) that selectively eliminate pathogenic bacteria, including resistant strains and biofilms, are attractive alternatives to antibiotics, also as a component of a new generation of antimicrobial wound dressings. AuresinePlus is a novel, engineered enzybiotic effective against Staphylococcus aureus—one of the most common pathogenic bacteria, found in infected wounds with a very high prevalence of antibiotic resistance. We took advantage of its potent lytic activity, selectivity, and safety to prepare a set of biodegradable PLGA/chitosan fibers generated by electrospinning. Our aim was to produce antimicrobial nonwovens to deliver enzybiotics directly to the infected wound and better control its release and activity. Three different methods of enzyme immobilization were tested: physical adsorption on the previously hydrolyzed surface, and covalent bonding formation using N-hydroxysuccinimide/N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (NHS/EDC) or glutaraldehyde (GA). The supramolecular structure and functional properties analysis revealed that the selected methods resulted in significant development of nanofibers surface topography resulting in an efficient enzybiotic attachment. Both physically adsorbed and covalently bound enzymes (by NHS/EDC method) exhibited prominent antibacterial activity. Here, we present the extensive comparison between methods for the effective attachment of the enzybiotic to the electrospun nonwovens to generate biomaterials effective against antibiotic-resistant strains. Our intention was to present a comprehensive proof-of-concept study for future antimicrobial wound dressing development. Słowa kluczowe: antibacterial wound dressings, enzybiotic, fibers functionalization, electrospun wound dressings, Staphylococcus aureus Afiliacje autorów:
Urbanek O. | - | IPPT PAN | Wysocka A. | - | inna afiliacja | Nakielski P. | - | IPPT PAN | Pierini F. | - | IPPT PAN | Jagielska E. | - | inna afiliacja | Sabała I. | - | Mossakowski Medical Research Centre, Polish Academy of Sciences (PL) |
| | 100p. |
8. |
Nakielski P., Pawłowska S., Rinoldi C., Ziai Y., De Sio L.♦, Urbanek O., Zembrzycki K., Pruchniewski M.♦, Lanzi M.♦, Salatelli E.♦, Calogero A.♦, Kowalewski T.A., Yarin A.L.♦, Pierini F., Multifunctional platform based on electrospun nanofibers and plasmonic hydrogel: a smart nanostructured pillow for near-Infrared light-driven biomedical applications,
ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.0c13266, Vol.12, No.49, pp.54328-54342, 2020Streszczenie: Multifunctional nanomaterials with the ability torespond to near-infrared (NIR) light stimulation are vital for thedevelopment of highly efficient biomedical nanoplatforms with apolytherapeutic approach. Inspired by the mesoglea structure ofjellyfish bells, a biomimetic multifunctional nanostructured pillowwith fast photothermal responsiveness for NIR light-controlled on-demand drug delivery is developed. We fabricate a nanoplatformwith several hierarchical levels designed to generate a series ofcontrolled, rapid, and reversible cascade-like structural changesupon NIR light irradiation. The mechanical contraction of thenanostructured platform, resulting from the increase of temper-ature to 42°C due to plasmonic hydrogel−light interaction, causesa rapid expulsion of water from the inner structure, passing through an electrospun membrane anchored onto the hydrogel core. Themutual effects of the rise in temperature and waterflow stimulate the release of molecules from the nanofibers. To expand thepotential applications of the biomimetic platform, the photothermal responsiveness to reach the typical temperature level forperforming photothermal therapy (PTT) is designed. The on-demand drug model penetration into pig tissue demonstrates theefficiency of the nanostructured platform in the rapid and controlled release of molecules, while the high biocompatibility confirmsthe pillow potential for biomedical applications based on the NIR light-driven multitherapy strategy. Słowa kluczowe: bioinspired materials, NIR-light responsive nanomaterials, multifunctional platforms, electrospun nanofibers, plasmonic hydrogel, photothermal-based polytherapy, on-demand drug delivery Afiliacje autorów:
Nakielski P. | - | IPPT PAN | Pawłowska S. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Ziai Y. | - | IPPT PAN | De Sio L. | - | Sapienza University of Rome (IT) | Urbanek O. | - | IPPT PAN | Zembrzycki K. | - | IPPT PAN | Pruchniewski M. | - | inna afiliacja | Lanzi M. | - | University of Bologna (IT) | Salatelli E. | - | University of Bologna (IT) | Calogero A. | - | Sapienza University of Rome (IT) | Kowalewski T.A. | - | IPPT PAN | Yarin A.L. | - | Technion - Israel Institute of Technology (IL) | Pierini F. | - | IPPT PAN |
| | 200p. |
9. |
Pierini F., Guglielmelli A.♦, Urbanek O., Nakielski P., Pezzi L.♦, Buda R.♦, Lanzi M.♦, Kowalewski T.A., De Sio L.♦, Thermoplasmonic‐activated hydrogel based dynamic light attenuator,
Advanced Optical Materials, ISSN: 2195-1071, DOI: 10.1002/adom.202000324, Vol.8, No.12, pp.2000324-1-7, 2020Streszczenie: This work describes the morphological, optical, and thermo‐optical properties of a temperature‐sensitive hydrogel poly(N‐isopropylacrylamide‐co‐N‐isopropylmethacrylamide) [P(NIPAm‐co‐NIPMAm]) film containing a specific amount of gold nanorods (GNRs). The light‐induced thermoplasmonic heating of GNRs is used to control the optical scattering of an initially transparent hydrogel film. A hydrated P(NIPAm‐co‐NIPMAm) film is optically clear at room temperature. When heated to temperatures over 37 °C via light irradiation with a resonant source (λ = 810 nm) to the GNRs, a reversible phase transition from a swollen hydrated state to a shrunken dehydrated state occurs. This phenomenon causes a drastic and reversible change in the optical transparency from a clear to an opaque state. A significant red shift (≈30 nm) of the longitudinal band can also be seen due to an increased average refractive index surrounding the GNRs. This change is in agreement with an ad hoc theoretical model which uses a modified Gans theory for ellipsoidal nanoparticles. Morphological analysis of the composite film shows the presence of well‐isolated and randomly dispersed GNRs. Thermo‐optical experiments demonstrate an all‐optically controlled light attenuator (65% contrast ratio) which can be easily integrated in several modern optical applications such as smart windows and light‐responsive optical attenuators. Słowa kluczowe: active plasmonics, gold nanorods, hydrogels, optical attenuators, optical transparency, plasmonic nanoparticles, polymers Afiliacje autorów:
Pierini F. | - | IPPT PAN | Guglielmelli A. | - | University of Calabria (IT) | Urbanek O. | - | IPPT PAN | Nakielski P. | - | IPPT PAN | Pezzi L. | - | inna afiliacja | Buda R. | - | Institute of Physical Chemistry, Polish Academy of Sciences (PL) | Lanzi M. | - | University of Bologna (IT) | Kowalewski T.A. | - | IPPT PAN | De Sio L. | - | Sapienza University of Rome (IT) |
| | 140p. |
10. |
Zaszczyńska A., Sajkiewicz P.Ł., Gradys A., Tymkiewicz R., Urbanek O., Kołbuk D., Influence of process-material conditions on the structure and biological properties of electrospun polyvinylidene fluoride fibers,
BULLETIN OF THE POLISH ACADEMY OF SCIENCES: TECHNICAL SCIENCES, ISSN: 0239-7528, DOI: 10.24425/bpasts.2020.133368, Vol.68, No.3, pp.627-633, 2020Streszczenie: Polyvinylidene fluoride (PVDF) is one of the most important piezoelectric polymers. Piezoelectricity in PVDF appears in polar β and ɣ phases. Piezoelectric fibers obtained by means of electrospinning may be used in tissue engineering (TE) as a smart analogue of the natural extracellular matrix (ECM). We present results showing the effect of rotational speed of the collecting drum on morphology, phase content and in vitro biological properties of PVDF nonwovens. Morphology and phase composition were analyzed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR), respectively. It was shown that increasing rotational speed of the collector leads to an increase in fiber orientation, reduction in fiber diameter and considerable increase of polar phase content, both b and g. In vitro cell culture experiments, carried out with the use of ultrasounds in order to generate electrical potential via piezoelectricity, indicate a positive effect of polar phases on fibroblasts. Our preliminary results demonstrate that piezoelectric PVDF scaffolds are promising materials for tissue engineering applications, particularly for neural tissue regeneration, where the electric potential is crucial. Słowa kluczowe: scaffolds, electrospinning, polyvinylidene fluoride, tissue engineering Afiliacje autorów:
Zaszczyńska A. | - | IPPT PAN | Sajkiewicz P.Ł. | - | IPPT PAN | Gradys A. | - | IPPT PAN | Tymkiewicz R. | - | IPPT PAN | Urbanek O. | - | IPPT PAN | Kołbuk D. | - | IPPT PAN |
| | 100p. |
11. |
Pawłowska S., Rinoldi C., Nakielski P., Ziai Y., Urbanek O., Li X.♦, Kowalewski T.A., Ding B.♦, Pierini F., Ultraviolet light‐assisted electrospinning of core–shell fully cross‐linked P(NIPAAm‐co‐NIPMAAm) hydrogel‐based nanofibers for thermally induced drug delivery self‐regulation,
Advanced Materials Interfaces, ISSN: 2196-7350, DOI: 10.1002/admi.202000247, Vol.7, No.12, pp.2000247-1-13, 2020Streszczenie: Body tissues and organs have complex functions which undergo intrinsic changes during medical treatments. For the development of ideal drug delivery systems, understanding the biological tissue activities is necessary to be able to design materials capable of changing their properties over time, on the basis of the patient's tissue needs. In this study, a nanofibrous thermal‐responsive drug delivery system is developed. The thermo‐responsivity of the system makes it possible to self‐regulate the release of bioactive molecules, while reducing the drug delivery at early stages, thus avoiding high concentrations of drugs which may be toxic for healthy cells. A co‐axial electrospinning technique is used to fabricate core–shell cross‐linked copolymer poly(N‐isopropylacrylamide‐co‐N‐isopropylmethacrylamide) (P(NIPAAm‐co‐NIPMAAm)) hydrogel‐based nanofibers. The obtained nanofibers are made of a core of thermo‐responsive hydrogel containing a drug model, while the outer shell is made of poly‐l‐lactide‐co‐caprolactone (PLCL). The custom‐made electrospinning apparatus enables the in situ cross‐linking of P(NIPAAm‐co‐NIPMAAm) hydrogel into a nanoscale confined space, which improves the electrospun nanofiber drug dosing process, by reducing its provision and allowing a self‐regulated release control. The mechanism of the temperature‐induced release control is studied in depth, and it is shown that the system is a promising candidate as a "smart" drug delivery platform. Słowa kluczowe: biomimetic nanomaterials, electrospun core–shell nanofibers, hierarchical nanostructures, smart drug delivery, thermo‐responsive hydrogels Afiliacje autorów:
Pawłowska S. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Nakielski P. | - | IPPT PAN | Ziai Y. | - | IPPT PAN | Urbanek O. | - | IPPT PAN | Li X. | - | Donghua University (CN) | Kowalewski T.A. | - | IPPT PAN | Ding B. | - | Donghua University (CN) | Pierini F. | - | IPPT PAN |
| | 100p. |
12. |
Kołbuk D., Heljak M.♦, Choińska E.♦, Urbanek O., Novel 3D hybrid nanofiber scaffolds for bone regeneration,
Polymers, ISSN: 2073-4360, DOI: 10.3390/polym12030544, Vol.12, No.3, pp.544-1-18, 2020Streszczenie: Development of hybrid scaffolds and their formation methods occupies an important place in tissue engineering. In this paper, a novel method of 3D hybrid scaffold formation is presented as well as an explanation of the differences in scaffold properties, which were a consequence of different crosslinking mechanisms. Scaffolds were formed from 3D freeze-dried gelatin and electrospun poly(lactide-co-glicolide) (PLGA) fibers in a ratio of 1:1 w/w. In order to enhance osteoblast proliferation, the fibers were coated with hydroxyapatite nanoparticles (HAp) using sonochemical processing. All scaffolds were crosslinked using an EDC/NHS solution. The scaffolds' morphology was imaged using scanning electron microscopy (SEM). The chemical composition of the scaffolds was analyzed using several methods. Water absorption and mass loss investigations proved a higher crosslinking degree of the hybrid scaffolds than a pure gelatin scaffold, caused by additional interactions between gelatin, PLGA, and HAp. Additionally, mechanical properties of the 3D hybrid scaffolds were higher than traditional hydrogels. In vitro studies revealed that fibroblasts and osteoblasts proliferated and migrated well on the 3D hybrid scaffolds, and also penetrated their structure during the seven days of the experiment. Słowa kluczowe: hybrid scaffolds, electrospinning, freeze-drying, gelatin, hydroxyapatite, sonochemical covering/grafting Afiliacje autorów:
Kołbuk D. | - | IPPT PAN | Heljak M. | - | Politechnika Warszawska (PL) | Choińska E. | - | Politechnika Warszawska (PL) | Urbanek O. | - | IPPT PAN |
| | 100p. |
13. |
Kołbuk D., Urbanek O., Denis P., Choińska E.♦, Sonochemical coating as an effective method of polymeric nonwovens functionalization,
Journal of Biomedical Materials Research Part A, ISSN: 1549-3296, DOI: 10.1002/jbm.a.36751, Vol.107, No.11, pp.2447-2457, 2019Streszczenie: A surface of polymeric nonwovens may be coated with various types of nanoparticles for medical applications, filtration, and so forth. However, quite often methods used for surface modification are difficult to scale up or are not applicable for polymers. In this article, we present one-step process enabling nonwovens functionalization. Poly(l-lactide-co-glicolide) (PLGA) nonwovens were prepared by electrospinning process and coated with hydroxyapatite nanoparticles (HAp) using ultrasonic processing. The effect of the process was evaluated with various techniques. HAp layer was successfully attached without loss of structural properties of HAp or PLGA nonwovens. The analysis confirmed the decrease of hydrophobicity of coated nonwoven, as well as its biocompatibility, making this material valuable from the perspective of medical applications. The sonochemical functionalization of polymeric nonwovens may be considered as an effective and economic method, enhancing surface properties of electrospun nonwovens for various applications. Słowa kluczowe: lectrospinning, fibrous composites, nanoparticles, surface modification, ultrasonic treatment Afiliacje autorów:
Kołbuk D. | - | IPPT PAN | Urbanek O. | - | IPPT PAN | Denis P. | - | IPPT PAN | Choińska E. | - | Politechnika Warszawska (PL) |
| | 100p. |
14. |
Urbanek O., Kołbuk D., Wróbel M.♦, Articular cartilage: new directions and barriers of scaffolds development – review,
International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2018.1452224, Vol.68, No.7, pp.396-410, 2019Streszczenie: Despite progress which has been made in recent years in the field of cell-based therapies or cell scaffolds for cartilage regeneration, a lot of work still needs to be done. Scaffolds remain a great base for tissue regeneration. However, proper implantation procedures or post-treatment still await development. In this review we summarize paths of cartilage treatment, especially focusing on cell scaffold design and manufacture. As well as the advantages and disadvantages of available or investigated methods and materials, especially focusing on cartilage scaffold design. We show the most promising directions and barriers in the creation of healthy tissue. Słowa kluczowe: cartilage regeneration, medical devices, scaffold development, tissue engineering Afiliacje autorów:
Urbanek O. | - | IPPT PAN | Kołbuk D. | - | IPPT PAN | Wróbel M. | - | Centre for Specialized Surgery (PL) |
| | 70p. |
15. |
Zaszczyńska A., Sajkiewicz P., Gradys A., Kołbuk D., Urbanek O., Cellular studies on piezoelectric polyvinylidene fluoride nanofibers subjected to ultrasounds stimulations,
ENGINEERING OF BIOMATERIALS / INŻYNIERIA BIOMATERIAŁÓW, ISSN: 1429-7248, Vol.22, No.153, pp.25-25, 2019 | | 20p. |
16. |
Pierini F., Nakielski P., Urbanek O., Pawłowska S., Lanzi M.♦, De Sio L.♦, Kowalewski T.A., Polymer-Based Nanomaterials for Photothermal Therapy: From Light-Responsive to Multifunctional Nanoplatforms for Synergistically Combined Technologies,
BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.8b01138, Vol.19, No.11, pp.4147-4167, 2018Streszczenie: Materials for the treatment of cancer have been studied comprehensively over the past few decades. Among the various kinds of biomaterials, polymer-based nanomaterials represent one of the most interesting research directions in nanomedicine because their controlled synthesis and tailored designs make it possible to obtain nanostructures with biomimetic features and outstanding biocompatibility. Understanding the chemical and physical mechanisms behind the cascading stimuli-responsiveness of smart polymers is fundamental for the design of multifunctional nanomaterials to be used as photothermal agents for targeted polytherapy. In this review, we offer an in-depth overview of the recent advances in polymer nanomaterials for photothermal therapy, describing the features of three different types of polymer-based nanomaterials. In each case, we systematically show the relevant benefits, highlighting the strategies for developing light-controlled multifunctional nanoplatforms that are responsive in a cascade manner and addressing the open issues by means of an inclusive state-of-the-art review. Moreover, we face further challenges and provide new perspectives for future strategies for developing novel polymeric nanomaterials for photothermally assisted therapies. Afiliacje autorów:
Pierini F. | - | IPPT PAN | Nakielski P. | - | IPPT PAN | Urbanek O. | - | IPPT PAN | Pawłowska S. | - | IPPT PAN | Lanzi M. | - | University of Bologna (IT) | De Sio L. | - | Sapienza University of Rome (IT) | Kowalewski T.A. | - | IPPT PAN |
| | 45p. |
17. |
Urbanek O., Pierini F., Choińska E.♦, Sajkiewicz P., Bil M.♦, Święszkowski W.♦, Effect of hydroxyapatite nanoparticles addition on structure properties of poly(L-lactide-co-glycolide) after gamma sterilization,
Polymer Composites, ISSN: 0272-8397, DOI: 10.1002/pc.24028, Vol.39, No.4, pp.1023-1031, 2018Streszczenie: Physical and chemical factors resulting from the sterilization methods may affect the structure and properties of the materials which undergo this procedure. Poly(l-lactide-co-glicolide) (PLGA) is commonly used for medical applications, but, due to its inadequate mechanical properties, it is not recommended for load-bearing applications. One of the methods for improving PLGA mechanical properties is addition of hydroxyapatite nanoparticles (nHAp). The aim of this study was to evaluate the effect of nanoparticles addition on PLGA structure and properties after gamma radiation. According to our results, reduction of the molecular mass caused by gamma radiation was lower for PLGA with nHAp addition. Differential scanning calorimetry (DSC) analysis indicates an increase of crystallinity caused both by nHAp and gamma radiation. The first phenomenon can be explained by heteronucleation, while the second one is most probably related to higher molecular mobility of degrading polymer. Moreover, addition of nanoparticles increases thermal stability and affects the Young's modulus changes after gamma radiation. Afiliacje autorów:
Urbanek O. | - | IPPT PAN | Pierini F. | - | IPPT PAN | Choińska E. | - | Politechnika Warszawska (PL) | Sajkiewicz P. | - | IPPT PAN | Bil M. | - | Politechnika Warszawska (PL) | Święszkowski W. | - | inna afiliacja |
| | 30p. |
18. |
Pierini F., Lanzi M.♦, Nakielski P., Pawłowska S., Urbanek O., Zembrzycki K., Kowalewski T.A., Single-Material Organic Solar Cells Based on Electrospun Fullerene-Grafted Polythiophene Nanofibers,
Macromolecules, ISSN: 0024-9297, DOI: 10.1021/acs.macromol.7b00857, Vol.50, No.13, pp.4972-4981, 2017Streszczenie: Highly efficient single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes were fabricated by incorporating electrospun one-dimensional (1D) nanostructures obtained from polymer chain stretching. Poly(3-alkylthiophene) chains were chemically tailored in order to reduce the side effects of charge recombination which severely affected SMOC photovoltaic performance. This enabled us to synthesize a donor–acceptor conjugated copolymer with high solubility, molecular weight, regioregularity, and fullerene content. We investigated the correlations among the active layer hierarchical structure given by the inclusion of electrospun nanofibers and the solar cell photovoltaic properties. The results indicated that SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%). The enhanced performance may be attributed to well-packed and properly oriented polymer chains. Overall, our work demonstrates that the active material structure optimization obtained by including electrospun nanofibers plays a pivotal role in the development of efficient SMOCs and suggests an interesting perspective for the improvement of copolymer-based photovoltaic device performance using an alternative pathway. Afiliacje autorów:
Pierini F. | - | IPPT PAN | Lanzi M. | - | University of Bologna (IT) | Nakielski P. | - | IPPT PAN | Pawłowska S. | - | IPPT PAN | Urbanek O. | - | IPPT PAN | Zembrzycki K. | - | IPPT PAN | Kowalewski T.A. | - | IPPT PAN |
| | 45p. |
19. |
Urbanek O., Sajkiewicz P., Pierini F., The effect of polarity in the electrospinning process on PCL/chitosan nanofibres' structure, properties and efficiency of surface modification,
POLYMER, ISSN: 0032-3861, DOI: 10.1016/j.polymer.2017.07.064, Vol.124, pp.168-175, 2017Streszczenie: The aim of this research was to study the effect of charge polarity applied to the spinning nozzle on the structure and properties of polycaprolactone/chitosan (PCL/CHT) blends, in particular the efficiency of further surface modification by chondroitin sulphate (CS). The observed differences in the morphology and properties of fibres formed at different polarities were interpreted in terms of molecular interactions occurring in the system. FTIR results indicate stronger PCL-chitosan interactions at negative polarity, resulting in lower PCL crystallinity and crystal size distribution determined by DSC, as well as lower wettability. The charge polarity influences PCL/CHT fibre morphology and tailors some of their properties, e.g. wettability, mechanical properties and the efficiency of surface modification. Better efficiency of CS attachment was observed at negative polarity using atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) is most probably related to higher chitosan content at the fibres' surface being attracted by the negative external potential. Słowa kluczowe: Polycaprolactone/chitosan nanofibres, Charge potential effect in electrospinning, Polycaprolactone-chitosan interactions Afiliacje autorów:
Urbanek O. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN | Pierini F. | - | IPPT PAN |
| | 40p. |
20. |
Urbanek O., Sajkiewicz P., Pierini F., Czerkies M., Kołbuk D., Structure and properties of polycaprolactone/chitosan nonwovens tailored by solvent systems,
Biomedical Materials, ISSN: 1748-6041, DOI: 10.1088/1748-605X/aa5647, Vol.12, No.1, pp.015020-1-12, 2017Streszczenie: Electrospinning of chitosan blends is a reasonable idea to prepare fibre mats for biomedical applications. Synthetic and natural components provide, for example, appropriate mechanical strength and biocompatibility, respectively. However, solvent characteristics and the polyelectrolyte nature of chitosan influence the spinnability of these blends. In order to compare the effect of solvent on polycaprolactone/chitosan fibres, two types of the most commonly used solvent systems were chosen, namely 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and acetic acid (AA)/formic acid (FA). Results obtained by various experimental methods clearly indicated the effect of the solvent system on the structure and properties of electrospun polycaprolactone/chitosan fibres. Viscosity measurements confirmed different polymer–solvent interactions. Various molecular interactions resulting in different macromolecular conformations of chitosan influenced its spinnability and properties. HFIP enabled fibres to be obtained whose average diameter was less than 250 nm while maintaining the brittle and hydrophilic character of the nonwoven, typical for the chitosan component. Spectroscopy studies revealed the formation of chitosan salts in the case of the AA/FA solvent system. Chitosan salts visibly influenced the structure and properties of the prepared fibre mats. The use of AA/FA caused a reduction of Young's modulus and wettability of the proposed blends. It was confirmed that wettability, mechanical properties and the antibacterial effect of polycaprolactone/chitosan fibres may be tailored by selecting an appropriate solvent system. The MTT cell proliferation assay revealed an increase of cytotoxicity to mouse fibroblasts in the case of 25% w/w of chitosan in electrospun nonwovens. Słowa kluczowe: chitosan, electrospinning, PCL/chitosan fibres, solvent system, chitosan salts Afiliacje autorów:
Urbanek O. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN | Pierini F. | - | IPPT PAN | Czerkies M. | - | IPPT PAN | Kołbuk D. | - | IPPT PAN |
| | 30p. |
21. |
Mayerberger E.A.♦, Urbanek O., McDaniel R.M.♦, Street R.M.♦, Barsoum M.W.♦, Schauer C.L.♦, Preparation and characterization of polymer-Ti3C2Tx(MXene) composite nanofibers produced via electrospinning,
JOURNAL OF APPLIED POLYMER SCIENCE, ISSN: 0021-8995, DOI: 10.1002/app.45295, Vol.134, No.37, pp.45295-1-7, 2017Streszczenie: MXene, a recently-discovered family of two-dimensional (2 D) transition metal carbides and/or nitrides, have attracted much interest because of their unique electrical, thermal, and mechanical properties. In this study, poly(acrylic acid) (PAA), polyethylene oxide (PEO), poly(vinyl alcohol) (PVA), and alginate/PEO were electrospun with delaminated Ti3C2 (MXene) flakes. The effect of small additions of delaminated Ti3C2 (1% w/w) on the structure and properties of the nanofibers were investigated and compared with those of the neat polymer nanofibers using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). Ti3C2 had an effect on the solution properties of the polymer and a greater effect on the average fiber diameter. The Ti3C2Tx/PEO solution exhibited the largest change in viscosity and conductivity with an 11% and 73.6% increase over the base polymer, respectively. X-ray diffractograms demonstrated a high degree of crystallization for Ti3C2/PEO and a slight decrease in crystallinity for Ti3C2/PVA. Słowa kluczowe: composite nanofibers, electrospinning, MXene Afiliacje autorów:
Mayerberger E.A. | - | Drexel University (US) | Urbanek O. | - | IPPT PAN | McDaniel R.M. | - | Drexel University (US) | Street R.M. | - | Drexel University (US) | Barsoum M.W. | - | Drexel University (US) | Schauer C.L. | - | Drexel University (US) |
| | 25p. |