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Stefaniuk M.♦, Gualda E.J.♦, Pawlowska M.♦, Legutko D.♦, Matryba P.♦, Koza P.♦, Konopka W.♦, Owczarek D.♦, Wawrzyniak M.♦, Loza-Alvarez P.♦, Kaczmarek L.♦, Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene,
Scientific Reports, ISSN: 2045-2322, DOI: 10.1038/srep28209, Vol.6, pp.28209-1-9, 2016 Abstract:
Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods. Finally, we demonstrate efficient imaging of the rat brain using light-sheet fluorescence microscopy. Affiliations:
| Stefaniuk M. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Gualda E.J. | - | Barcelona Institute of Science and Technology (ES) | | Pawlowska M. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Legutko D. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Matryba P. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Koza P. | - | other affiliation | | Konopka W. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Owczarek D. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Wawrzyniak M. | - | Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL) | | Loza-Alvarez P. | - | Barcelona Institute of Science and Technology (ES) | | Kaczmarek L. | - | other affiliation |
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