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Brun-Cosme-Bruny M.♦, Pernet L.♦, Błoński S., Zaremba D., Fraboulet S.♦, Dolega M.E.♦, Microfluidic system for in vitro epithelial folding and calcium waves induction,
STAR Protocols, ISSN: 2666-1667, DOI: 10.1016/j.xpro.2022.101683, Vol.3, No.4, pp.101683-101683, 2022 Abstract:
Epithelial folding is a fundamental process where initially flat monolayers transform into functional 3D structures. This protocol details fabrication steps for a polycarbonate microfluidic platform which enables triggering epithelial folds that recapitulate stereotypical cell shape changes and folding-associated mechanical stresses. We describe the steps for cell seeding to form a monolayer on the chip, and subsequent approach to trigger calcium waves in the epithelial monolayer through local epithelial deformation. Lastly, we outline quantitative analysis steps of the epithelial response. Affiliations:
| Brun-Cosme-Bruny M. | - | other affiliation | | Pernet L. | - | other affiliation | | Błoński S. | - | IPPT PAN | | Zaremba D. | - | IPPT PAN | | Fraboulet S. | - | other affiliation | | Dolega M.E. | - | Université Grenoble Alpes (FR) |
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