1. |
Gloc M.♦, Przybysz S.♦, Dulnik J., Kołbuk-Konieczny D., Wachowski M.♦, Kosturek R.♦, Ślęzak T.♦, Krawczyńska A.♦, Ciupiński ♦, A Comprehensive Study of a Novel Explosively Hardened Pure Titanium Alloy for Medical Applications,
Materials, ISSN: 1996-1944, DOI: 10.3390/ma16227188, Vol.16, No.22, pp.7188--1-19, 2023Streszczenie: Pure titanium is gaining increasing interest due to its potential use in dental and orthopedic applications. Due to its relatively weak mechanical parameters, a limited number of components manufactured from pure titanium are available on the market. In order to improve the mechanical parameters of pure titanium, manufacturers use alloys containing cytotoxic vanadium and aluminum. This paper presents unique explosive hardening technology that can be used to strengthen pure titanium parameters. The analysis confirms that explosive induced α-ω martensitic transformation and crystallographic anisotropy occurred due to the explosive pressure. The mechanical properties related to residual stresses are very nonuniform. The corrosion properties of the explosive hardened pure titanium test do not change significantly compared to nonhardened titanium. The biocompatibility of all the analyzed samples was confirmed in several tests. The morphology of bone cells does not depend on the titanium surface phase composition and crystallographic orientation. Słowa kluczowe: explosive hardening, pure titanium, bioimplants, titanium alloys Afiliacje autorów:
Gloc M. | - | Politechnika Warszawska (PL) | Przybysz S. | - | Institute of High Pressure Physics, Polish Academy of Sciences (PL) | Dulnik J. | - | IPPT PAN | Kołbuk-Konieczny D. | - | IPPT PAN | Wachowski M. | - | inna afiliacja | Kosturek R. | - | inna afiliacja | Ślęzak T. | - | inna afiliacja | Krawczyńska A. | - | Politechnika Warszawska (PL) | Ciupiński | - | Politechnika Warszawska (PL) |
| | 140p. |
2. |
El-Okaily Mohamed S.♦, Mostafa Amany A.♦, Dulnik J., Denis P., Sajkiewicz P.Ł., Mahmoud Azza A.♦, Dawood R.♦, Maged A.♦, Nanofibrous Polycaprolactone Membrane with Bioactive Glass and Atorvastatin for Wound Healing: Preparation and Characterization,
Pharmaceutics, ISSN: 1999-4923, DOI: 10.3390/pharmaceutics15071990, Vol.15, No.7, pp.1990-1-19, 2023Streszczenie: Skin wound healing is one of the most challenging processes for skin reconstruction, especially after severe injuries. In our study, nanofiber membranes were prepared for wound healing using an electrospinning process, where the prepared nanofibers were made of different weight ratios of polycaprolactone and bioactive glass that can induce the growth of new tissue. The membranes showed smooth and uniform nanofibers with an average diameter of 118 nm. FTIR and XRD results indicated no chemical interactions of polycaprolactone and bioactive glass and an increase in polycaprolactone crystallinity by the incorporation of bioactive glass nanoparticles. Nanofibers containing 5% w/w of bioactive glass were selected to be loaded with atorvastatin, considering their best mechanical properties compared to the other prepared nanofibers (3, 10, and 20% w/w bioactive glass). Atorvastatin can speed up the tissue healing process, and it was loaded into the selected nanofibers using a dip-coating technique with ethyl cellulose as a coating polymer. The study of the in vitro drug release found that atorvastatin-loaded nanofibers with a 10% coating polymer revealed gradual drug release compared to the non-coated nanofibers and nanofibers coated with 5% ethyl cellulose. Integration of atorvastatin and bioactive glass with polycaprolactone nanofibers showed superior wound closure results in the human skin fibroblast cell line. The results from this study highlight the ability of polycaprolactone-bioactive glass-based fibers loaded with atorvastatin to stimulate skin wound healing. Słowa kluczowe: nanofibers, polycaprolactone, bioactive glass, coating, wound healing Afiliacje autorów:
El-Okaily Mohamed S. | - | inna afiliacja | Mostafa Amany A. | - | inna afiliacja | Dulnik J. | - | IPPT PAN | Denis P. | - | IPPT PAN | Sajkiewicz P.Ł. | - | IPPT PAN | Mahmoud Azza A. | - | inna afiliacja | Dawood R. | - | inna afiliacja | Maged A. | - | inna afiliacja |
| | 140p. |
3. |
Wasyłeczko M.♦, Remiszewska E.♦, Sikorska W.♦, Dulnik J., Chwojnowski A.♦, Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers,
Molecules, ISSN: 1420-3049, DOI: 10.3390/molecules28073195, Vol.28, No.7, pp.3195-1-28, 2023Streszczenie: In recent years, one of the main goals of cartilage tissue engineering has been to find appropriate scaffolds for hyaline cartilage regeneration, which could serve as a matrix for chondrocytes or stem cell cultures. The study presents three types of scaffolds obtained from a blend of polyethersulfone (PES) and polyurethane (PUR) by a combination of wet-phase inversion and salt-leaching methods. The nonwovens made of gelatin and sodium chloride (NaCl) were used as precursors of macropores. Thus, obtained membranes were characterized by a suitable structure. The top layers were perforated, with pores over 20 µm, which allows cells to enter the membrane. The use of a nonwoven made it possible to develop a three-dimensional network of interconnected macropores that is required for cell activity and mobility. Examination of wettability (contact angle, swelling ratio) showed a hydrophilic nature of scaffolds. The mechanical test showed that the scaffolds were suitable for knee joint applications (stress above 10 MPa). Next, the scaffolds underwent a degradation study in simulated body fluid (SBF). Weight loss after four weeks and changes in structure were assessed using scanning electron microscopy (SEM) and MeMoExplorer Software, a program that estimates the size of pores. The porosity measurements after degradation confirmed an increase in pore size, as expected. Hydrolysis was confirmed by Fourier-transform infrared spectroscopy (FT-IR) analysis, where the disappearance of ester bonds at about 1730 cm −1 wavelength is noticeable after degradation. The obtained results showed that the scaffolds meet the requirements for cartilage tissue engineering membranes and should undergo further testing on an animal model. Słowa kluczowe: articular cartilage, cartilage tissue engineering, hydrolysis process, materials for scaffolds, partly degradable scaffolds, polyethersulfone–polyurethane scaffolds, polyurethane degradation, regenerative medicine, scaffold requirements, tissue engineering Afiliacje autorów:
Wasyłeczko M. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Remiszewska E. | - | inna afiliacja | Sikorska W. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Dulnik J. | - | IPPT PAN | Chwojnowski A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) |
| | 140p. |
4. |
Wasyłeczko M.♦, Krysiak Z.J.♦, Łukowska E.♦, Gruba M.♦, Sikorska W.♦, Kruk A.♦, Dulnik J., Czubak J.♦, Chwojnowski A.♦, Three-dimensional scaffolds for bioengineering of cartilage tissue,
Biocybernetics and Biomedical Engineering, ISSN: 0208-5216, DOI: 10.1016/j.bbe.2022.03.004, Vol.42, No.2, pp.494-511, 2022Streszczenie: The cartilage tissue is neither supplied with blood nor innervated, so it cannot heal by itself. Thus, its reconstruction is highly challenging and requires external support. Cartilage diseases are becoming more common due to the aging population and obesity. Among young people, it is usually a post-traumatic complication. Slight cartilage damage leads to the spontaneous formation of fibrous tissue, not resistant to abrasion and stress, resulting in cartilage degradation and the progression of the disease. For these reasons, cartilage regeneration requires further research, including use of new type of biomaterials for scaffolds. This paper shows cartilage characteristics within its most frequent problems and treatment strategies, including a promising method that combines scaffolds and human cells. Structure and material requirements, manufacturing methods, and commercially available scaffolds were described. Also, the comparison of poly(L-lactide) (PLLA) and polyethersulfone (PES) 3D membranes obtained by a phase inversion method using nonwovens as a pore-forming additives were reported. The scaffolds' structure and the growth ability of human chondrocytes were compared. Scaffolds' structure, cells morphology, and protein presence in the membranes were examined with a scanning electron microscope. The metabolic activity of cells was tested with the MTT assay. The structure of the scaffolds and the growth capacity of human chondrocytes were compared. Obtained results showed higher cell activity and protein content for PES scaffolds than for PLLA. The PES membrane had better mechanical properties (e.g. ripping), greater chondrocytes proliferation, and thus a better secretion of proteins which build up the cartilage structure. Słowa kluczowe: 3D-scaffolds, membrane structure, polyethersulfone, poly(L-lactide), chondrocyte culture, cartilage regeneration Afiliacje autorów:
Wasyłeczko M. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Krysiak Z.J. | - | inna afiliacja | Łukowska E. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Gruba M. | - | Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL) | Sikorska W. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Kruk A. | - | Politechnika Warszawska (PL) | Dulnik J. | - | IPPT PAN | Czubak J. | - | Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL) | Chwojnowski A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) |
| | 140p. |
5. |
Dulnik J., Jeznach O., Sajkiewicz P., A Comparative Study of Three Approaches to Fibre’s Surface Functionalization,
Journal of Functional Biomaterials, ISSN: 2079-4983, DOI: 10.3390/jfb13040272, Vol.13, No.4, pp.272-1-23, 2022Streszczenie: Polyester-based scaffolds are of research interest for the regeneration of a wide spectrum of tissues. However, there is a need to improve scaffold wettability and introduce bioactivity. Surface modification is a widely studied approach for improving scaffold performance and maintaining appropriate bulk properties. In this study, three methods to functionalize the surface of the poly(lactide-co-ε-caprolactone) PLCL fibres using gelatin immobilisation were compared. Hydrolysis, oxygen plasma treatment, and aminolysis were chosen as activation methods to introduce carboxyl (-COOH) and amino (-NH2) functional groups on the surface before gelatin immobilisation. To covalently attach the gelatin, carbodiimide coupling was chosen for hydrolysed and plasma-treated materials, and glutaraldehyde crosslinking was used in the case of the aminolysed samples. Materials after physical entrapment of gelatin and immobilisation using carbodiimide coupling without previous activation were prepared as controls. The difference in gelatin amount on the surface, impact on the fibres morphology, molecular weight, and mechanical properties were observed depending on the type of modification and applied parameters of activation. It was shown that hydrolysis influences the surface of the material the most, whereas plasma treatment and aminolysis have an effect on the whole volume of the material. Despite this difference, bulk mechanical properties were affected for all the approaches. All materials were completely hydrophilic after functionalization. Cytotoxicity was not recognized for any of the samples. Gelatin immobilisation resulted in improved L929 cell morphology with the best effect for samples activated with hydrolysis and plasma treatment. Our study indicates that the use of any surface activation method should be limited to the lowest concentration/reaction time that enables subsequent satisfactory functionalization and the decision should be based on a specific function that the final scaffold material has to perform. Słowa kluczowe: surface activation,functionalization,electrospun fibres,hydrolysis,plasma,aminolysis Afiliacje autorów:
Dulnik J. | - | IPPT PAN | Jeznach O. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN |
| | 100p. |
6. |
Dulnik J., Sajkiewicz P., Crosslinking of gelatin in bicomponent electrospun fibers,
Materials, ISSN: 1996-1944, DOI: 10.3390/ma14123391, Vol.14, No.12, pp.3391-1-13, 2021Streszczenie: Four chemical crosslinking methods were used in order to prevent gelatin leaching in an aqueous environment, from bicomponent polycaprolactone/gelatin (PCL/Gt) nanofibers electrospun from an alternative solvent system. A range of different concentrations and reaction times were employed to compare genipin, 1-(3-dimethylaminopropyl)-N’-ethylcarbodimide hydrochloride/N-hydroxysuccinimide (EDC/NHS), 1,4-butanediol diglycidyl ether (BDDGE), and transglutaminase. The objective was to optimize and find the most effective method in terms of reaction time and solution concentration, that at the same time provides satisfactory gelatin crosslinking degree and ensures good morphology of the fibers, even after 24 h in aqueous medium in 37 °C. The series of experiments demonstrated that, out of the four compared crosslinking methods, EDC/NHS was able to yield satisfactory results with the lowest concentrations and the shortest reaction times. Słowa kluczowe: crosslinking, gelatin, nanofibers, biodegradable polymers, electrospinning Afiliacje autorów:
Dulnik J. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN |
| | 140p. |
7. |
Wasyleczko M.♦, Sikorska W.♦, Przytulska M.♦, Dulnik J., Chwojnowski A.♦, Polyester membranes as 3D scaffolds for cell culture,
Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2021.26658, Vol.214, pp.181-193, 2021Streszczenie: The study presents two types of three-dimensional membranes made of the biodegradable copolymer. They were obtained by the wet-phase inversion method using different solvent and pore precursors. In one case, a nonwoven made of gelatin and polyvinylpyrrolidone (PVP) as precursors of macropores and small pores, respectively, were used. In the second case, PVP nonwovens and Pluronic were used properly for macro- and micro-pores. As the material, a biodegradable poly(L-lactide-co-ε-caprolactone) is composed of 30% ε-caprolactone and 70% poly(L-lactic acid) was used. Depending on the pore precursors, different membrane structures were obtained. The morphology of pores was studied using the MeMoExplorer™, an advanced software designed for computer analysis of the scanning electron microscopy images. The scaffolds were degraded in phosphate-buffered saline and Hank’s balanced salt solutions at 37°C. Moreover, the porosity of the membranes before and after hydrolysis was calculated. Słowa kluczowe: 3D scaffolds, poly(L-lactide-co-ε-caprolactone), porosity of membrane, phase inversion method, degradation of scaffolds Afiliacje autorów:
Wasyleczko M. | - | inna afiliacja | Sikorska W. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Przytulska M. | - | inna afiliacja | Dulnik J. | - | IPPT PAN | Chwojnowski A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) |
| | 100p. |
8. |
Niemczyk-Soczyńska B., Dulnik J., Jeznach O., Kołbuk D., Sajkiewicz P., Shortening of electrospun PLLA fibers by ultrasonication,
Micron, ISSN: 0968-4328, DOI: 10.1016/j.micron.2021.103066, Vol.145, pp.103066-1-8, 2021Streszczenie: This research work is aimed at studying the effect of ultrasounds on the effectiveness of fiber fragmentation by taking into account the type of sonication medium, processing time, and various PLLA molecular weights. Fragmentation was followed by an appropriate filtration in order to decrease fibers length distribution. It was evidenced by fiber length determination using SEM that the fibers are shortened after ultrasonic treatment, and the effectiveness of shortening depends on the two out of three investigated parameters, mostly on the sonication medium, and processing time. The gel permeation chromatography (GPC) confirmed that such ultrasonic treatment does not change the polymers' molecular weight. Our results allowed to optimize the ultrasonic fragmentation procedure of electrospun fibers while preliminary viscosity measurements of fibers loaded into hydrogel confirmed their potential in further use as fillers for injectable hydrogels for regenerative medicine applications. Słowa kluczowe: electrospinning, ultrasonication, short fibers, polymers Afiliacje autorów:
Niemczyk-Soczyńska B. | - | IPPT PAN | Dulnik J. | - | IPPT PAN | Jeznach O. | - | IPPT PAN | Kołbuk D. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN |
| | 100p. |
9. |
Wrzecionek M.♦, Bandzerewicz A.♦, Dutkowska E.♦, Dulnik J., Denis P., Gadomska-Gajadhur A.♦, Poly(glycerol citrate)-polylactide nonwovens toward tissue engineering applications,
Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.5407, Vol.32, No.10, pp.3955-3966, 2021Streszczenie: In 2002, Robert Langer proposed that new polyester for tissue engineering should have good mechanical properties followed by: covalent bonding (as crosslinking) and hydrogen-bonding interactions; and should be elastic like rubber materials due to three-dimensional network structure. Considering these hypotheses, a polyester made of glycerol and citric acid was designed in this work. Poly(glycerol citrate) should be attractive for tissue engineering because both glycerol and citric acid, taking part in natural human metabolic pathways; and due to the reactant's functionality, 3D networks should be produced easily. Moreover, the reagents are cheap, available, and often used in the food and pharmaceutical industries. In this work, poly(glycerol citrate) was synthesized and then used with PLA for creating porous nonwovens by electrospinning. Produced materials were tested for possible application in the field of tissue engineering. The obtained materials have properties similar to collagen fibers, but still, require refinement for medical applications. Słowa kluczowe: electrospinning, poly(glycerol citrate), polylactide, tissue engineering Afiliacje autorów:
Wrzecionek M. | - | Politechnika Warszawska (PL) | Bandzerewicz A. | - | Politechnika Warszawska (PL) | Dutkowska E. | - | Politechnika Warszawska (PL) | Dulnik J. | - | IPPT PAN | Denis P. | - | IPPT PAN | Gadomska-Gajadhur A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) |
| | 70p. |
10. |
Gadomska‐Gajadhur A.♦, Kruk A.♦, Dulnik J., Chwojnowski A.♦, New polyester biodegradable scaffolds for chondrocyte culturing: preparation, properties, and biological activity,
JOURNAL OF APPLIED POLYMER SCIENCE, ISSN: 0021-8995, DOI: 10.1002/app.50089, pp.e50089-1-14, 2020Streszczenie: An innovative modification of the wet inversion phase method, consisting in the use of a polymer nano‐nonwoven as a nonclassic pore precursor. Mechanical properties of the obtained scaffolds were determined, their hydrophilic properties (serum absorbability) were tested, and the content of residues of materials used in the scaffold preparation was determined. Nontoxicity of the developed scaffolds toward T lymphocyte cells was proved. Cultures of primary chondrocytes were obtained successfully. It was proved that an addition of a polymer nano‐nonwoven changes the properties of the scaffolds favorably in respect of their subsequent application in tissue engineering. Słowa kluczowe: cartilage regeneration, chondrocytes, nano-nonwoven, polyvinylpyrrolidone, T lymphocytes Afiliacje autorów:
Gadomska‐Gajadhur A. | - | inna afiliacja | Kruk A. | - | Politechnika Warszawska (PL) | Dulnik J. | - | IPPT PAN | Chwojnowski A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) |
| | 70p. |
11. |
Gadomska‐Gajadhur A.♦, Kruk A.♦, Ruśkowski P.♦, Sajkiewicz P., Dulnik J., Chwojnowski A.♦, Original method of imprinting pores in scaffolds for tissue engineering,
Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.5091, pp.1-13, 2020Streszczenie: Results of the preparation of biodegradable porous scaffolds using an original modification of a wet phase inversion method were presented. Influence of gelatin non‐woven as a non‐classic pore precursor and polyvinylpyrrolidone, Pluronic as classic pore precursors on the structure of obtained scaffolds was analyzed. It was shown that the addition of gelatin non‐wovens enables the preparation of scaffolds, which allow for the growth of cells (size, distribution, and shape of pores). Mechanical properties of the obtained cell scaffolds were determined. The influence of pore precursors on mass absorption of scaffolds against isopropanol and plasma was investigated. Interaction of scaffolds with a T‐lymphocyte line (Jurkat) and with fibroblasts (L929) was investigated. Obtained scaffolds are not cytotoxic and can be used as implants, for example, the regeneration of cartilage tissue. Słowa kluczowe: cell cultures, cytotoxic, fibroblasts, imprinted scaffolds Afiliacje autorów:
Gadomska‐Gajadhur A. | - | inna afiliacja | Kruk A. | - | Politechnika Warszawska (PL) | Ruśkowski P. | - | Politechnika Warszawska (PL) | Sajkiewicz P. | - | IPPT PAN | Dulnik J. | - | IPPT PAN | Chwojnowski A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) |
| | 70p. |
12. |
Kruk A.♦, Gadomska-Gajadhur A.♦, Dulnik J., Ruśkowski P.♦, The influence of the molecular weight of polymer on the morphology, functional properties and L929 fibroblasts growth on polylactide membranes for tissue engineering,
International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2020.1798440, pp.1-13, 2020Streszczenie: The main goal of tissue engineering (TE) is supporting the regeneration of damaged tissues that are difficult to regenerate. The experimental results of the preparation of semi-permeable membranes for cell cultures are presented. The effect of the PLA molecular weight and addition of pore precursors on the morphology of the membranes was studied. The pore precursor of choice was polyvinylpyrrolidone (PVP). It was found that semi-permeable membranes for application in tissue engineering can be prepared with polylactides of molecular weight more significant than 37,000 g/mol. Moreover, it was observed that the growth of the molecular weight of the polymer, the porosity, the size of the pores, the Young modulus and maximum tensile increased. Additionally, to obtain a better morphology of the membranes, PVP should be added to the polymeric solution. Positive growth of L929 fibroblast cells on the obtained scaffolds was shown. Słowa kluczowe: biodegradable polymers, cell cultures, L929 fibroblasts, polylactide, scaffolds, tissue engineering Afiliacje autorów:
Kruk A. | - | Politechnika Warszawska (PL) | Gadomska-Gajadhur A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Dulnik J. | - | IPPT PAN | Ruśkowski P. | - | Politechnika Warszawska (PL) |
| | 70p. |
13. |
Yakymechko Y.♦, Lutsyuk I.♦, Jaskulski R., Dulnik J., Kropyvnytska T.♦, The effect of vibro-activation time on the properties of highly active calcium hydroxide,
Buildings, ISSN: 2075-5309, DOI: 10.3390/buildings10060111, Vol.10, No.6, pp.111-1-8, 2020Streszczenie: The results of studying the effect of the vibration processing time on the size of calcium hydroxide particles are given. The physicochemical processes affecting the size and morphology of calcium hydroxide particles have been studied. A stage-by-stage mechanism of the process of the carbonation of lime, depending on its specific surface, is established. The results show that the optimal period for the vibration treatment of lime to obtain the most active material is 20 min. A longer period of vibration results in the merging of particles into larger agglomerates. Słowa kluczowe: lime, portlandite, vibration treatment, carbonation, crystallization Afiliacje autorów:
Yakymechko Y. | - | Politechnika Warszawska (PL) | Lutsyuk I. | - | inna afiliacja | Jaskulski R. | - | IPPT PAN | Dulnik J. | - | IPPT PAN | Kropyvnytska T. | - | inna afiliacja |
| | 70p. |
14. |
Kruk A.♦, Gadomska-Gajadhur A.♦, Rykaczewska I.♦, Dulnik J., Ruśkowski P.♦, Synoradzki L.♦, Influence of liquid pore precursors on morphology and mechanical properties of 3D scaffolds obtained by dry inversion phase method,
Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34200, Vol.107, No.4, pp.1079-1087, 2019Streszczenie: Polyester 3D scaffolds were obtained by dry inversion phase method. The influence of a polymer and liquid pore precursor type on the 3D scaffolds morphology, porosity and mechanical properties was tested. Polymers and precursors forming a porous structure were identified. It was found that 3D scaffolds having the most preferable structure for cell cultures were obtained from polylactide with the addition of n‐butanol as the liquid pore precursor. Słowa kluczowe: liquid pore precursors, mechanical properties, dry inversion phase method, 3D scaffolds Afiliacje autorów:
Kruk A. | - | Politechnika Warszawska (PL) | Gadomska-Gajadhur A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Rykaczewska I. | - | Politechnika Warszawska (PL) | Dulnik J. | - | IPPT PAN | Ruśkowski P. | - | Politechnika Warszawska (PL) | Synoradzki L. | - | Politechnika Warszawska (PL) |
| | 140p. |
15. |
Dulnik J., Kołbuk D., Denis P., Sajkiewicz P., The effect of a solvent on cellular response to PCL/gelatin and PCL/collagen electrospun nanofibres,
EUROPEAN POLYMER JOURNAL, ISSN: 0014-3057, DOI: 10.1016/j.eurpolymj.2018.05.010, Vol.104, pp.147-156, 2018Streszczenie: Bicomponent polycaprolactone/gelatin and polycaprolactone/collagen fibres were formed by electrospinning using two kinds of solvents: a representative of commonly used solvents with this polymer composition, highly toxic hexafluoroisopropanol (HFIP) and alternative, less harmful one, the mixture of acetic (AA) and formic (FA) acids. Both material types were subjected to investigations of structure and in-vitro cellular activity. Viscosity and Fourier transform infrared spectroscopy (FTIR) measurements shown that the type of solvent used influences the structure of solution and conformation of polymer molecules. In-vitro quantitative tests as well as cell culture morphology observations proved that materials electrospun with the use of 'green' solvents can yield similar results to those obtained by made with toxic ones. Slightly better cellular response to materials electrospun from HFIP can be explained by relatively well dispersed components within the fibre and more expanded conformation of molecules, resulting in better exposition of RGD (Arg-Gly-Asp) binding sites to cells' integrin receptors. Słowa kluczowe: Cellular tests, Electrospinning, Biopolymers, Viscosity, Solvents Afiliacje autorów:
Dulnik J. | - | IPPT PAN | Kołbuk D. | - | IPPT PAN | Denis P. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN |
| | 35p. |
16. |
Kruk A.♦, Gadomska-Gajadhur A.♦, Dulnik J., Rykaczewska I.♦, Ruśkowski P.♦, Sebai A.♦, Synoradzki L.♦, Ocena właściwości użytkowych rusztowań komórkowych o strukturze gąbczastej oraz wzrostu na nich fibroblastów,
POLIMERY, ISSN: 0032-2725, DOI: 10.14314/polimery.2018.4.3, Vol.63, No.4, pp.18-22, 2018Streszczenie: Zbadano wpływ dodatku ciekłych prekursorów porów na morfologię, porowatość i właściwości mechaniczne polilaktydowych rusztowań komórkowych. Rusztowania otrzymano metodą mokrej inwersji faz w wariancie freeze extraction. Oceniono cytotoksyczność wybranych rusztowań w stosunku do fibroblastów mysich oraz ich przydatność do hodowli komórkowych. Wykazano, że dodatek prekursora porów dopolilaktydu korzystnie zmienia morfologię wytworzonych rusztowań, jednocześnie pogarszając ich wytrzymałość mechaniczną. Stwierdzono, że polilaktydowe rusztowania komórkowe z powodzeniem mogą być wykorzystywane do hodowli komórkowych. Słowa kluczowe: usztowania komórkowe, polilaktyd, hodowle komórkowe, fibroblasty Afiliacje autorów:
Kruk A. | - | Politechnika Warszawska (PL) | Gadomska-Gajadhur A. | - | Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL) | Dulnik J. | - | IPPT PAN | Rykaczewska I. | - | Politechnika Warszawska (PL) | Ruśkowski P. | - | Politechnika Warszawska (PL) | Sebai A. | - | Wroclaw University of Science and Technology (PL) | Synoradzki L. | - | Politechnika Warszawska (PL) |
| | 15p. |
17. |
Dulnik J., Denis P., Sajkiewicz P., Kołbuk D., Choińska E.♦, Biodegradation of bicomponent PCL/gelatin and PCL/collagen nanofibers electrospun from alternative solvent system,
Polymer Degradation and Stability, ISSN: 0141-3910, DOI: 10.1016/j.polymdegradstab.2016.05.022, Vol.130, pp.10-21, 2016Streszczenie: Bicomponent polycaprolactone/gelatin and polycaprolactone/collagen nanofibers formed by electrospinning using various solvents were subjected to biodegradation and compared. Hexafluoroisopropanol (HFIP) was used as a reference solvent, while the second, alternative solvent system was the mixture of acetic acid (AA) with formic acid (FA). Biodegradation of investigated materials was manifested mainly by the gelatin leaching, including collagen which is indeed denaturated to gelatin during electrospinning, leading to nanofibers erosion. There was no molecular degradation of PCL during 90 days of biodegradation procedure as deduced from no change in the elongation stress at break. The rate of biopolymer leaching was very fast from all materials during the first 24 h of biodegradation, being related to surface leaching, followed by a slower rate leaching from deeper material layers. Mass measurements showed much faster biopolymer leaching from nanofibers electrospun from AA/FA than from HFIP because of strongly emulsive nature of the solution in the former case. Irrespective of the solvent used, the leaching rate increased with initial content of gelatin. The analysis of Young modulus during biodegradation indicated complex mechanism of changes, including biopolymer mass loss, increase of PCL crystallinity and partial gelatin renaturation. Słowa kluczowe: Bicomponent nanofibers, Biodegradation, Biopolymer Afiliacje autorów:
Dulnik J. | - | IPPT PAN | Denis P. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN | Kołbuk D. | - | IPPT PAN | Choińska E. | - | Politechnika Warszawska (PL) |
| | 35p. |
18. |
Jarząbek D.M., Chmielewski M.♦, Dulnik J., Strojny-Nędza A.♦, The Influence of the Particle Size on the Adhesion Between Ceramic Particles and Metal Matrix in MMC Composites,
Journal of Materials Engineering and Performance, ISSN: 1059-9495, DOI: 10.1007/s11665-016-2107-3, Vol.25, No.8, pp.3139-3145, 2016Streszczenie: This study investigated the influence of the particle size on the adhesion force between ceramic particles and metal matrix in ceramic-reinforced metal matrix composites. The Cu-Al2O3 composites with 5 vol.% of ceramic phase were prepared by a powder metallurgy process. Alumina oxide powder as an electrocorundum (Al2O3) powder with different particle sizes, i.e., fine powder <3 µm and coarse powder of 180 µm was used as a reinforcement. Microstructural investigations included analyses using scanning electron microscopy with an integrated EDS microanalysis system and transmission microscopy. In order to measure the adhesion force (interface strength), we prepared the microwires made of the investigated materials and carried out the experiments with the use of the self-made tensile tester. We have observed that the interface strength is higher for the sample with coarse particles and is equal to 74 ± 4 MPa and it is equal to 68 ± 3 MPa for the sample with fine ceramic particles. Słowa kluczowe: adhesion, interface strength, metal matrix composites, nanocomposites, tensile test Afiliacje autorów:
Jarząbek D.M. | - | IPPT PAN | Chmielewski M. | - | Institute of Electronic Materials Technology (PL) | Dulnik J. | - | IPPT PAN | Strojny-Nędza A. | - | Institute of Electronic Materials Technology (PL) |
| | 20p. |
19. |
Denis P., Dulnik J., Sajkiewicz P., Electrospinning and Structure of Bicomponent Polycaprolactone/Gelatin Nanofibers Obtained Using Alternative Solvent System,
International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2014.945208, Vol.64, No.7, pp.354-364, 2015Streszczenie: Bicomponent polycaprolactone/gelatin (PCL/Gt) nanofibers were successfully formed for the first time by electrospinning using a novel polymer–solvent system with solvents being alternative to the commonly used toxic solvents like fluorinated alcohols. The mixture of acetic acid (AA) with formic acid (FA; 90:10) was applied. Stable electrospinning was possible despite the fact the mixture of PCL and gelatin in AA/FA solvent showed emulsive structure. From the practical perspective, there is no doubt that it is possible to obtain PCL/Gt fibers using AA/FA mixture with morphology similar to that for fibers spun from hexafluoroisopropanol (HFIP) solutions. Słowa kluczowe: Alternative solvents, electrospinning, gelatin, nanofibers, polycaprolactone, structure Afiliacje autorów:
Denis P. | - | IPPT PAN | Dulnik J. | - | IPPT PAN | Sajkiewicz P. | - | IPPT PAN |
| | 25p. |