1. |
Nakielski P., Rybak D., Jezierska-Woźniak K.♦, Rinoldi C., Sinderewicz E.♦, Staszkiewicz-Chodor J.♦, Haghighat Bayan M.A., Czelejewska W.♦, Urbanek-Świderska O., Kosik-Kozioł A., Barczewska M.♦, Skomorowski M.♦, Holak P.♦, Lipiński S.♦, Maksymowicz W.♦, Pierini F., Minimally invasive intradiscal delivery of BM-MSCs via fibrous microscaffold carriers,
ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.3c11710, pp.1-16, 2023Abstract: Current treatments of degenerated intervertebral discs often provide only temporary relief or address specific causes, necessitating the exploration of alternative therapies. Cell-based regenerative approaches showed promise in many clinical trials, but
limitations such as cell death during injection and a harsh disk environment hinder their effectiveness. Injectable microscaffolds offer a solution by providing a supportive microenvironment for cell delivery and enhancing bioactivity. This study evaluated the
safety and feasibility of electrospun nanofibrous microscaffolds modified with chitosan (CH) and chondroitin sulfate (CS) for treating degenerated NP tissue in a large animal model. The microscaffolds facilitated cell attachment and acted as an effective delivery system, preventing cell leakage under a high disc pressure. Combining microscaffolds with bone marrow-derived mesenchymal stromal cells demonstrated no cytotoxic effects and proliferation over the entire microscaffolds. The administration of cells attached to microscaffolds into the NP positively influenced the regeneration process of the intervertebral disc. Injectable poly(L-lactide-co-glycolide) and poly(L-lactide) microscaffolds enriched with CH or CS, having a fibrous structure, showed the potential to promote intervertebral disc regeneration. These features collectively address critical challenges in the fields of tissue engineering and regenerative medicine, particularly in the context of intervertebral disc degeneration. Keywords: microscaffolds,cell carriers,injectable biomaterials,intervertebral disc,laser micromachining,electrospinning Affiliations:
Nakielski P. | - | IPPT PAN | Rybak D. | - | IPPT PAN | Jezierska-Woźniak K. | - | other affiliation | Rinoldi C. | - | IPPT PAN | Sinderewicz E. | - | other affiliation | Staszkiewicz-Chodor J. | - | other affiliation | Haghighat Bayan M.A. | - | IPPT PAN | Czelejewska W. | - | other affiliation | Urbanek-Świderska O. | - | IPPT PAN | Kosik-Kozioł A. | - | IPPT PAN | Barczewska M. | - | University of Warmia and Mazury in Olsztyn (PL) | Skomorowski M. | - | other affiliation | Holak P. | - | other affiliation | Lipiński S. | - | other affiliation | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Pierini F. | - | IPPT PAN |
| |
2. |
Nakielski P., Rinoldi C., Pruchniewski M.♦, Pawłowska S., Gazińska M.♦, Strojny B.♦, Rybak D., Jezierska-Woźniak K.♦, Urbanek O., Denis P., Sinderewicz E.♦, Czelejewska W.♦, Staszkiewicz-Chodor J.♦, Grodzik M.♦, Ziai Y., Barczewska M.♦, Maksymowicz W.♦, Pierini F., Laser-assisted fabrication of injectable nanofibrous cell carriers,
Small, ISSN: 1613-6810, DOI: 10.1002/smll.202104971, Vol.18, No.2, pp.2104971-1-18, 2022Abstract: The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications. Affiliations:
Nakielski P. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Pruchniewski M. | - | other affiliation | Pawłowska S. | - | IPPT PAN | Gazińska M. | - | other affiliation | Strojny B. | - | other affiliation | Rybak D. | - | IPPT PAN | Jezierska-Woźniak K. | - | other affiliation | Urbanek O. | - | IPPT PAN | Denis P. | - | IPPT PAN | Sinderewicz E. | - | other affiliation | Czelejewska W. | - | other affiliation | Staszkiewicz-Chodor J. | - | other affiliation | Grodzik M. | - | other affiliation | Ziai Y. | - | IPPT PAN | Barczewska M. | - | University of Warmia and Mazury in Olsztyn (PL) | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Pierini F. | - | IPPT PAN |
| |
3. |
Rinoldi C., Lanzi M.♦, Fiorelli R.♦, Nakielski P., Zembrzycki K., Kowalewski T., Urbanek O., Jezierska-Woźniak K.♦, Maksymowicz W.♦, Camposeo A.♦, Bilewicz R.♦, Pisignano D.♦, Sanai N.♦, Pierini F., Pierini F., Three-dimensional printable conductive semi-interpenetrating polymer network hydrogel for neural tissue applications,
BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.1c00524, Vol.22, No.7, pp.3084-3098, 2021Abstract: Intrinsically conducting polymers (ICPs) are widely used to fabricate biomaterials; their application in neural tissue engineering, however, is severely limited because of their hydrophobicity and insufficient mechanical properties. For these reasons, soft conductive polymer hydrogels (CPHs) are recently developed, resulting in a water-based system with tissue-like mechanical, biological, and electrical properties. The strategy of incorporating ICPs as a conductive component into CPHs is recently explored by synthesizing the hydrogel around ICP chains, thus forming a semi-interpenetrating polymer network (semi-IPN). In this work, a novel conductive semi-IPN hydrogel is designed and synthesized. The hybrid hydrogel is based on a poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide) hydrogel where polythiophene is introduced as an ICP to provide the system with good electrical properties. The fabrication of the hybrid hydrogel in an aqueous medium is made possible by modifying and synthesizing the monomers of polythiophene to ensure water solubility. The morphological, chemical, thermal, electrical, electrochemical, and mechanical properties of semi-IPNs were fully investigated. Additionally, the biological response of neural progenitor cells and mesenchymal stem cells in contact with the conductive semi-IPN was evaluated in terms of neural differentiation and proliferation. Lastly, the potential of the hydrogel solution as a 3D printing ink was evaluated through the 3D laser printing method. The presented results revealed that the proposed 3D printable conductive semi-IPN system is a good candidate as a scaffold for neural tissue applications. Affiliations:
Rinoldi C. | - | IPPT PAN | Lanzi M. | - | University of Bologna (IT) | Fiorelli R. | - | other affiliation | Nakielski P. | - | IPPT PAN | Zembrzycki K. | - | IPPT PAN | Kowalewski T. | - | IPPT PAN | Grippo V. | - | other affiliation | Urbanek O. | - | IPPT PAN | Jezierska-Woźniak K. | - | other affiliation | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Camposeo A. | - | other affiliation | Bilewicz R. | - | other affiliation | Pisignano D. | - | other affiliation | Sanai N. | - | other affiliation | Pierini F. | - | IPPT PAN |
| |