Institute of Fundamental Technological Research
Polish Academy of Sciences

Partners

Michał Pruchniewski, MSc


Recent publications
1.  Rybak D., Rinoldi C., Nakielski P., Du J., Haghighat Bayan M.A., Zargarian S. S., Pruchniewski M., Li X., Strojny-Cieślak B., Ding B., Pierini F., Injectable and self-healable nano-architectured hydrogel for NIR-light responsive chemo- and photothermal bacterial eradication, JOURNAL OF MATERIALS CHEMISTRY B , ISSN: 2050-7518, DOI: 10.1039/D3TB02693K, pp.1-21, 2024

Abstract:
Hydrogels with multifunctional properties activated at specific times have gained significant attention in the biomedical field. As bacterial infections can cause severe complications that negatively impact wound repair, herein, we present the development of a stimuli-responsive, injectable, and in situ-forming hydrogel with antibacterial, self-healing, and drug-delivery properties. In this study, we prepared a Pluronic F-127 (PF127) and sodium alginate (SA)-based hydrogel that can be targeted to a specific tissue via injection. The PF127/SA hydrogel was incorporated with polymeric short-filaments (SFs) containing an anti-inflammatory drug – ketoprofen, and stimuli-responsive polydopamine (PDA) particles. The hydrogel, after injection, could be in situ gelated at the body temperature, showing great in vitro stability and self-healing ability after 4 h of incubation. The SFs and PDA improved the hydrogel injectability and compressive strength. The introduction of PDA significantly accelerated the KET release under near-infrared light exposure and extended its release validity period. The excellent composites’ photo-thermal performance led to antibacterial activity against representative Gram-positive and Gram-negative bacteria, resulting in 99.9% E. coli and S. aureus eradication after 10 min of NIR light irradiation. In vitro, fibroblast L929 cell studies confirmed the materials’ biocompatibility and paved the way toward further in vivo and clinical application of the system for chronic wound treatments.

Affiliations:
Rybak D. - IPPT PAN
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Du J. - University of California (US)
Haghighat Bayan M.A. - IPPT PAN
Zargarian S. S. - IPPT PAN
Pruchniewski M. - other affiliation
Li X. - Donghua University (CN)
Strojny-Cieślak B. - other affiliation
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
2.  Pruchniewski M., Sawosz E., Sosnowska-Ławnicka M., Ostrowska A., Łojkowski M., Koczoń P., Nakielski P., Kutwin M., Jaworski S., Strojny-Cieślak B., Nanostructured graphene oxide enriched with metallic nanoparticles as a biointerface to enhance cell adhesion through mechanosensory modifications, NANOSCALE, ISSN: 2040-3364, DOI: 10.1039/d3nr03581f, Vol.15, No.46, pp.18639-18659, 2023

Abstract:
Nanostructuring is a process involving surface manipulation at the nanometric level, which improves the mechanical and biological properties of biomaterials. Specifically, it affects the mechanotransductive perception of the microenvironment of cells. Mechanical force conversion into an electrical or chemical signal contributes to the induction of a specific cellular response. The relationship between the cells and growth surface induces a biointerface-modifying cytophysiology and consequently a therapeutic effect. In this study, we present the fabrication of graphene oxide (GO)-based nanofilms decorated with metallic nanoparticles (NPs) as potential coatings for biomaterials. Our investigation showed the effect of decorating GO with metallic NPs for the modification of the physicochemical properties of nanostructures in the form of nanoflakes and nanofilms. A comprehensive biocompatibility screening panel revealed no disturbance in the metabolic activity of human fibroblasts (HFFF2) and bone marrow stroma cells (HS-5) cultivated on the GO nanofilms decorated with gold and copper NPs, whereas a significant cytotoxic effect of the GO nanocomplex decorated with silver NPs was demonstrated. The GO nanofilm decorated with gold NPs beneficially managed early cell adhesion as a result of the transient upregulation of α1β5 integrin expression, acceleration of cellspreading, and formation of elongated filopodia. Additionally, the cells, sensing the substrate derived from the nanocomplex enriched with gold NPs, showed reduced elasticity and altered levels of vimentin expression. In the future, GO nanocomplexes decorated with gold NPs can be incorporated in the structure of architecturally designed biomimetic biomaterials as biocompatible nanostructuring agents with proadhesive properties.

Affiliations:
Pruchniewski M. - other affiliation
Sawosz E. - Warsaw University of Life Sciences (PL)
Sosnowska-Ławnicka M. - other affiliation
Ostrowska A. - other affiliation
Łojkowski M. - other affiliation
Koczoń P. - other affiliation
Nakielski P. - IPPT PAN
Kutwin M. - Warsaw University of Life Sciences (PL)
Jaworski S. - Warsaw University of Life Sciences (PL)
Strojny-Cieślak B. - other affiliation
3.  Nakielski P., Rinoldi C., Pruchniewski M., Pawłowska S., Gazińska M., Strojny B., Rybak D., Jezierska-Woźniak K., Urbanek O., Denis P., Sinderewicz E., Czelejewska W., Staszkiewicz-Chodor J., Grodzik M., Ziai Y., Barczewska M., Maksymowicz W., Pierini F., Laser-assisted fabrication of injectable nanofibrous cell carriers, Small, ISSN: 1613-6810, DOI: 10.1002/smll.202104971, Vol.18, No.2, pp.2104971-1-18, 2022

Abstract:
The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications.

Affiliations:
Nakielski P. - IPPT PAN
Rinoldi C. - IPPT PAN
Pruchniewski M. - other affiliation
Pawłowska S. - IPPT PAN
Gazińska M. - other affiliation
Strojny B. - other affiliation
Rybak D. - IPPT PAN
Jezierska-Woźniak K. - other affiliation
Urbanek O. - IPPT PAN
Denis P. - IPPT PAN
Sinderewicz E. - other affiliation
Czelejewska W. - other affiliation
Staszkiewicz-Chodor J. - other affiliation
Grodzik M. - other affiliation
Ziai Y. - IPPT PAN
Barczewska M. - University of Warmia and Mazury in Olsztyn (PL)
Maksymowicz W. - University of Warmia and Mazury in Olsztyn (PL)
Pierini F. - IPPT PAN
4.  Nakielski P., Pawłowska S., Rinoldi C., Ziai Y., De Sio L., Urbanek O., Zembrzycki K., Pruchniewski M., Lanzi M., Salatelli E., Calogero A., Kowalewski T.A., Yarin A.L., Pierini F., Multifunctional platform based on electrospun nanofibers and plasmonic hydrogel: a smart nanostructured pillow for near-Infrared light-driven biomedical applications, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.0c13266, Vol.12, No.49, pp.54328-54342, 2020

Abstract:
Multifunctional nanomaterials with the ability torespond to near-infrared (NIR) light stimulation are vital for thedevelopment of highly efficient biomedical nanoplatforms with apolytherapeutic approach. Inspired by the mesoglea structure ofjellyfish bells, a biomimetic multifunctional nanostructured pillowwith fast photothermal responsiveness for NIR light-controlled on-demand drug delivery is developed. We fabricate a nanoplatformwith several hierarchical levels designed to generate a series ofcontrolled, rapid, and reversible cascade-like structural changesupon NIR light irradiation. The mechanical contraction of thenanostructured platform, resulting from the increase of temper-ature to 42°C due to plasmonic hydrogel−light interaction, causesa rapid expulsion of water from the inner structure, passing through an electrospun membrane anchored onto the hydrogel core. Themutual effects of the rise in temperature and waterflow stimulate the release of molecules from the nanofibers. To expand thepotential applications of the biomimetic platform, the photothermal responsiveness to reach the typical temperature level forperforming photothermal therapy (PTT) is designed. The on-demand drug model penetration into pig tissue demonstrates theefficiency of the nanostructured platform in the rapid and controlled release of molecules, while the high biocompatibility confirmsthe pillow potential for biomedical applications based on the NIR light-driven multitherapy strategy.

Keywords:
bioinspired materials, NIR-light responsive nanomaterials, multifunctional platforms, electrospun nanofibers, plasmonic hydrogel, photothermal-based polytherapy, on-demand drug delivery

Affiliations:
Nakielski P. - IPPT PAN
Pawłowska S. - IPPT PAN
Rinoldi C. - IPPT PAN
Ziai Y. - IPPT PAN
De Sio L. - Sapienza University of Rome (IT)
Urbanek O. - IPPT PAN
Zembrzycki K. - IPPT PAN
Pruchniewski M. - other affiliation
Lanzi M. - University of Bologna (IT)
Salatelli E. - University of Bologna (IT)
Calogero A. - Sapienza University of Rome (IT)
Kowalewski T.A. - IPPT PAN
Yarin A.L. - Technion-Israel Institute of Technology (IL)
Pierini F. - IPPT PAN

Conference abstracts
1.  Nakielski P., Rinoldi C., Pruchniewski M., Rybak D., Jezierska-Woźniak K., Gazińska M., Strojny B., Grodzik M., Maksymowicz W., Pierini F., Injectable nanofibrous microscaffolds, EHDAES, European Symposium on Electrohydrodynamic Atomization and Electrospinning, 2022-04-27/04-29, Napoli (IT), pp.1, 2022
2.  Nakielski P., Rinoldi C., Pruchniewski M., Rybak D., Jezierska-Woźniak K., Gazińska M., Strojny B., Grodzik M., Maksymowicz W., Pierini F., Injectable nanofibrous microscaffolds for cell and drug delivery, TERMIS-EU 2022, Tissue Engineering and Regenerative Medicine International Society European Chapter Conference 2022, 2022-06-28/07-01, Kraków (PL), pp.1, 2022
3.  Nakielski P., Rinoldi C., Pruchniewski M., Rybak D., Urbanek O., Jezierska- Woźniak K., Grodzik M., Maksymowicz W., Pierini F., Injectable microscaffolds for IVD regeneration, 2022 eCM20: Cartilage and Disc Repair and Regeneration, 2022-06-15/06-18, Davos (CH), pp.33-33, 2022
4.  Pierini F., Nakielski P., Pawłowska S., Rinoldi C., Ziai Y., Urbanek-Świderska O., De Sio L., Calogero A., Lanzi M., Zembrzycki K., Pruchniewski M., Salatelli E., Kowalewski T.A., Yarin A., Nature-inspired smart drug delivery platforms based on electrospun nanofibers and plasmonic hydrogels for near-infrared light-controlled polytherapy, Polymer Connect, Polymer Science and Composite Materials Conference, 2020-02-26/02-28, LISBON (PT), pp.7, 2020

Patents
Filing No./Date
Filing Publication
Autors
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
PCT/PL2022050004
2022-02-03
WO/2022/177454
2022-08-25
Nakielski P., Pawłowska S., Pruchniewski M., Urbanek-Świderska O., Pierini F.
A method for obtaining injectable biocompatible drug delivery vehicles, cell carriers or combinations thereof, in the form of microscaffolds, an injectable composition containing said vehicles, and its applications
WO, Instytut Podstawowych Problemów Techniki PAN
-
-
-
437078
2021-02-22
BUP 35/2022
2022-08-29
Nakielski P., Pawłowska S., Pruchniewski M., Urbanek-Świderska O., Pierini F.
Method for preparing an injectable biocompatible carrier of drugs, cells or combinations thereof, in the form of microforms, an injectable composition containing the said carriers, and its use
PL, Instytut Podstawowych Problemów Techniki PAN
244645
WUP 08/2024
2024-02-19



Category A Plus

IPPT PAN

logo ippt            Pawińskiego 5B, 02-106 Warsaw
  +48 22 826 12 81 (central)
  +48 22 826 98 15
 

Find Us

mapka
© Institute of Fundamental Technological Research Polish Academy of Sciences 2024