Institute of Fundamental Technological Research
Polish Academy of Sciences

Staff

Anna Zakrzewska, MSc

Department of Biosystems and Soft Matter (ZBiMM)
Division of Functional Polymer Nanomaterials (PZNP)
position: PhD Student
PhD student
telephone: (+48) 22 826 12 81 ext.: 422
room: 227
e-mail:
ORCID: 0000-0002-1764-8554

Recent publications
1.  Shah Syed A., Sohail M., Nakielski P., Rinoldi C., Zargarian Seyed S., Kosik-Kozioł A., Yasamin Z., Ali Haghighat Bayan M., Zakrzewska A., Rybak D., Bartolewska M., Pierini F., Integrating Micro- and Nanostructured Platforms and Biological Drugs to Enhance Biomaterial-Based Bone Regeneration Strategies, BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.4c01133, pp.A-W, 2024

Abstract:
Bone defects resulting from congenital anomalies and trauma pose significant clinical challenges for orthopedics surgeries, where bone tissue engineering (BTE) aims to address these challenges by repairing defects that fail to heal spontaneously. Despite numerous advances, BTE still faces several challenges, i.e., difficulties in detecting and tracking implanted cells, high costs, and regulatory approval hurdles. Biomaterials promise to revolutionize bone grafting procedures, heralding a new era of regenerative medicine and advancing patient outcomes worldwide. Specifically, novel bioactive biomaterials have been developed that promote cell adhesion, proliferation, and differentiation and have osteoconductive and osteoinductive characteristics, stimulating tissue regeneration and repair, particularly in complex skeletal defects caused by trauma, degeneration, and neoplasia. A wide array of biological therapeutics for bone regeneration have emerged, drawing from the diverse spectrum of gene therapy, immune cell interactions, and RNA molecules. This review will provide insights into the current state and potential of future strategies for bone regeneration.

Affiliations:
Shah Syed A. - IPPT PAN
Sohail M. - other affiliation
Nakielski P. - IPPT PAN
Rinoldi C. - IPPT PAN
Zargarian Seyed S. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Yasamin Z. - IPPT PAN
Ali Haghighat Bayan M. - IPPT PAN
Zakrzewska A. - IPPT PAN
Rybak D. - IPPT PAN
Bartolewska M. - IPPT PAN
Pierini F. - IPPT PAN
2.  Ziai Y., Rinoldi C., Petronella F., Zakrzewska A., De Sio L., Pierini F., Lysozyme-sensitive plasmonic hydrogel nanocomposite for colorimetric dry-eye inflammation biosensing, NANOSCALE, ISSN: 2040-3364, DOI: 10.1039/d4nr01701c, Vol.16, No.28, pp.13492-13502, 2024

Abstract:
Detection of lysozyme levels in ocular fluids is considered crucial for diagnosing and monitoring various health and eye conditions, including dry-eye syndrome. Hydrogel-based nanocomposites have been demonstrated to be one of the most promising platforms for fast and accurate sensing of different biomolecules. In this work, hydrogel, electrospun nanofibers, and plasmonic nanoparticles are combined to fabricate a sensitive and easy-to-use biosensor for lysozyme. Poly(L-lactide-co-caprolactone) (PLCL) nanofibers were covered with silver nanoplates (AgNPls), providing a stable plasmonic platform, where a poly(N-isopropylacrylamide)-based (PNIPAAm) hydrogel layer allows mobility and good integration of the biomolecules. By integrating these components, the platform can also exhibit a colorimetric response to the concentration of lysozyme, allowing for easy and non-invasive monitoring. Quantitative biosensing operates on the principle of localized surface plasmon resonance (LSPR) induced by plasmonic nanoparticles. Chemical, structural, thermal, and optical characterizations were performed on each platform layer, and the platform's ability to detect lysozyme at concentrations relevant to those found in tears of patients with dry-eye syndrome and other related diseases was investigated by colorimetry and UV-Vis spectroscopy. This biosensor's sensitivity and rapid response time, alongside the easy detection by the naked eye, make it a promising tool for early diagnosis and treatment monitoring of eye diseases.

Affiliations:
Ziai Y. - IPPT PAN
Rinoldi C. - IPPT PAN
Petronella F. - other affiliation
Zakrzewska A. - IPPT PAN
De Sio L. - other affiliation
Pierini F. - IPPT PAN
3.  Haghighat Bayan M.A., Rinoldi C., Rybak D., Zargarian S.S., Zakrzewska A., Miler O., Põhako-Palu K., Zhang S., Stobnicka-Kupiec A., Górny Rafał L., Nakielski P., Kogermann K., De Sio L., Ding B., Pierini F., Engineering surgical face masks with photothermal and photodynamic plasmonic nanostructures for enhancing filtration and on-demand pathogen eradication, Biomaterials Science, ISSN: 2047-4849, DOI: 10.1039/d3bm01125a, Vol.12, No.4, pp.949-963, 2024

Abstract:
The shortage of face masks and the lack of antipathogenic functions has been significant since the recent pandemic's inception. Moreover, the disposal of an enormous number of contaminated face masks not only carries a significant environmental impact but also escalates the risk of cross-contamination. This study proposes a strategy to upgrade available surgical masks into antibacterial masks with enhanced particle and bacterial filtration. Plasmonic nanoparticles can provide photodynamic and photothermal functionalities for surgical masks. For this purpose, gold nanorods act as on-demand agents to eliminate pathogens on the surface of the masks upon near-infrared light irradiation. Additionally, the modified masks are furnished with polymer electrospun nanofibrous layers. These electrospun layers can enhance the particle and bacterial filtration efficiency, not at the cost of the pressure drop of the mask. Consequently, fabricating these prototype masks could be a practical approach to upgrading the available masks to alleviate the environmental toll of disposable face masks.

Affiliations:
Haghighat Bayan M.A. - IPPT PAN
Rinoldi C. - IPPT PAN
Rybak D. - IPPT PAN
Zargarian S.S. - IPPT PAN
Zakrzewska A. - IPPT PAN
Miler O. - IPPT PAN
Põhako-Palu K. - other affiliation
Zhang S. - other affiliation
Stobnicka-Kupiec A. - other affiliation
Górny Rafał L. - other affiliation
Nakielski P. - IPPT PAN
Kogermann K. - other affiliation
De Sio L. - Sapienza University of Rome (IT)
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
4.  Zargarian S., Zakrzewska A., Kosik-Kozioł A., Bartolewska M., Shah S., Li X., Su Q., Petronella F., Marinelli M., De Sio L., Lanzi M., Ding B., Pierini F., Advancing resource sustainability with green photothermal materials: Insights from organic waste-derived and bioderived sources, nanotechnology reviews, ISSN: 2191-9097, DOI: 10.1515/ntrev-2024-0100, Vol.13, No.1, pp.20240100-1-39, 2024

Abstract:
Recently, there has been a surge of interest in developing new types of photothermal materials driven by the ongoing demand for efficient energy conversion, environmental concerns, and the need for sustainable solutions. However, many existing photothermal materials face limitations such as high production costs or narrow absorption bands, hindering their widespread application. In response to these challenges, researchers have redirected their focus toward harnessing the untapped potential of organic waste-derived and bioderived materials. These materials, with photothermal properties derived from their intrinsic composition or transformative processes, offer a sustainable and cost-effective alternative. This review provides an extended categorization of organic waste-derived and bioderived materials based on their origin. Additionally, we investigate the mechanisms underlying the photothermal properties of these materials. Key findings highlight their high photothermal efficiency and versatility in applications such as water and energy harvesting, desalination, biomedical applications, deicing, waste treatment, and environmental remediation. Through their versatile utilization, they demonstrate immense potential in fostering sustainability and support the transition toward a greener and more resilient future. The authors’ perspective on the challenges and potentials of platforms based on these materials is also included, highlighting their immense potential for real-world implementation.

Keywords:
photothermal materials, organic waste valorization, bioderived materials

Affiliations:
Zargarian S. - IPPT PAN
Zakrzewska A. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Bartolewska M. - IPPT PAN
Shah S. - IPPT PAN
Li X. - Donghua University (CN)
Su Q. - other affiliation
Petronella F. - other affiliation
Marinelli M. - other affiliation
De Sio L. - other affiliation
Lanzi M. - University of Bologna (IT)
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
5.  Zargarian S., Kupikowska-Stobba B., Kosik-Kozioł A., Bartolewska M., Zakrzewska A., Rybak D., Bochenek K., Osial M., Pierini F., Light-responsive biowaste-derived and bio-inspired textiles: Dancing between bio-friendliness and antibacterial functionality, Materials Today Chemistry, ISSN: 2468-5194, DOI: 10.1016/j.mtchem.2024.102281, Vol.41, pp.102281-1-15, 2024

Abstract:
Functional antibacterial textiles fabricated from a hybrid of organic waste-derived and bio-inspired materials offer sustainable solutions for preventing microbial infections. In this work, we developed a novel antibacterial textile created through the valorization of spent coffee grounds (SCG). Electrospinning and electrospraying techniques were employed to integrate the biowaste within a polymeric nanofiber matrix, ensuring uniform particle distribution and providing structural support for enhanced applicability. Modification with polydopamine (PDA) significantly enhanced the textile's photothermal performance. Specific attention was paid to understanding the relation between temperature change and key variables, including the surrounding liquid volume, textile layer stacking, and applied laser power. Developed platforms demonstrated excellent photothermal stability. While the SCG-based textile demonstrated exceptional biocompatibility, the PDA-modified textile effectively eradicated Staphylococcus aureus (S. aureus) under near-infrared (NIR) irradiation. The developed textiles in our work demonstrate a dynamic balance between biocompatibility and on-demand antibacterial functionality, offering adaptable solutions in accordance with the desired application.

Keywords:
Organic waste valorization, Spent coffee grounds, Micro-nanostructured textiles, Bio-inspired photothermal agents, Polydopamine, Antibacterial textiles

Affiliations:
Zargarian S. - IPPT PAN
Kupikowska-Stobba B. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Bartolewska M. - IPPT PAN
Zakrzewska A. - IPPT PAN
Rybak D. - IPPT PAN
Bochenek K. - IPPT PAN
Osial M. - IPPT PAN
Pierini F. - IPPT PAN
6.  Ziai Y., Lanzi M., Rinoldi C., Zargarian S.S., Zakrzewska A., Kosik-Kozioł A., Nakielski P., Pierini F., Developing strategies to optimize the anchorage between electrospun nanofibers and hydrogels for multi-layered plasmonic biomaterials, Nanoscale Advances, ISSN: 2516-0230, DOI: 10.1039/d3na01022h, Vol.6, No.4, pp.1246-1258, 2024

Abstract:
Polycaprolactone (PCL), a recognized biopolymer, has emerged as a prominent choice for diverse biomedical endeavors due to its good mechanical properties, exceptional biocompatibility, and tunable properties. These attributes render PCL a suitable alternative biomaterial to use in biofabrication, especially the electrospinning technique, facilitating the production of nanofibers with varied dimensions and functionalities. However, the inherent hydrophobicity of PCL nanofibers can pose limitations. Conversely, acrylamide-based hydrogels, characterized by their interconnected porosity, significant water retention, and responsive behavior, present an ideal matrix for numerous biomedical applications. By merging these two materials, one can harness their collective strengths while potentially mitigating individual limitations. A robust interface and effective anchorage during the composite fabrication are pivotal for the optimal performance of the nanoplatforms. Nanoplatforms are subject to varying degrees of tension and physical alterations depending on their specific applications. This is particularly pertinent in the case of layered nanostructures, which require careful consideration to maintain structural stability and functional integrity in their intended applications. In this study, we delve into the influence of the fiber dimensions, orientation and surface modifications of the nanofibrous layer and the hydrogel layer's crosslinking density on their intralayer interface to determine the optimal approach. Comprehensive mechanical pull-out tests offer insights into the interfacial adhesion and anchorage between the layers. Notably, plasma treatment of the hydrophobic nanofibers and the stiffness of the hydrogel layer significantly enhance the mechanical effort required for fiber extraction from the hydrogels, indicating improved anchorage. Furthermore, biocompatibility assessments confirm the potential biomedical applications of the proposed nanoplatforms.

Affiliations:
Ziai Y. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Rinoldi C. - IPPT PAN
Zargarian S.S. - IPPT PAN
Zakrzewska A. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Nakielski P. - IPPT PAN
Pierini F. - IPPT PAN
7.  Rinoldi C., Ziai Y., Zargarian S.S., Nakielski P., Zembrzycki K., Haghighat Bayan M.A., Zakrzewska A., Fiorelli R., Lanzi M., Kostrzewska-Księżyk A., Czajkowski R., Kublik E., Kaczmarek L., Pierini F., In Vivo Chronic Brain Cortex Signal Recording Based on a Soft Conductive Hydrogel Biointerface, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.2c17025, Vol.15, No.5, pp.6283-6296, 2023

Abstract:
In neuroscience, the acquisition of neural signals from the brain cortex is crucial to analyze brain processes, detect neurological disorders, and offer therapeutic brain–computer interfaces. The design of neural interfaces conformable to the brain tissue is one of today’s major challenges since the insufficient biocompatibility of those systems provokes a fibrotic encapsulation response, leading to an inaccurate signal recording and tissue damage precluding long-term/permanent implants. The design and production of a novel soft neural biointerface made of polyacrylamide hydrogels loaded with plasmonic silver nanocubes are reported herein. Hydrogels are surrounded by a silicon-based template as a supporting element for guaranteeing an intimate neural-hydrogel contact while making possible stable recordings from specific sites in the brain cortex. The nanostructured hydrogels show superior electroconductivity while mimicking the mechanical characteristics of the brain tissue. Furthermore, in vitro biological tests performed by culturing neural progenitor cells demonstrate the biocompatibility of hydrogels along with neuronal differentiation. In vivo chronic neuroinflammation tests on a mouse model show no adverse immune response toward the nanostructured hydrogel-based neural interface. Additionally, electrocorticography acquisitions indicate that the proposed platform permits long-term efficient recordings of neural signals, revealing the suitability of the system as a chronic neural biointerface.

Keywords:
brain−machine interface,conductive hydrogels,nanostructured biomaterials,in vitro and in vivo biocompatibility,long-term neural recording

Affiliations:
Rinoldi C. - IPPT PAN
Ziai Y. - IPPT PAN
Zargarian S.S. - IPPT PAN
Nakielski P. - IPPT PAN
Zembrzycki K. - IPPT PAN
Haghighat Bayan M.A. - IPPT PAN
Zakrzewska A. - IPPT PAN
Fiorelli R. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Kostrzewska-Księżyk A. - other affiliation
Czajkowski R. - other affiliation
Kublik E. - other affiliation
Kaczmarek L. - other affiliation
Pierini F. - IPPT PAN
8.  Zangoli M., Monti F., Zanelli A., Marinelli M., Flammini S., Spallacci N., Zakrzewska A., Lanzi M., Salatelli E., Pierini F., Di Maria F., Multifunctional Photoelectroactive Materials for Optoelectronic Applications Based on Thieno[3,4-b]pyrazines and Thieno[1,2,5]thiadiazoles, Chemistry - A European Journal, ISSN: 0947-6539, DOI: 10.1002/chem.202303590, pp.1-18, 2023

Abstract:
In this study, we introduce a novel family of symmetrical thiophene-based small molecules with a Donor–Acceptor–Donor structure. These compounds feature three different acceptor units: benzo[c][1,2,5]thiadiazole (Bz), thieno[3,4-b]pyrazine (Pz), and thieno[1,2,5]thiadiazole (Tz), coupled with electron donor units based on a carbazole-thiophene derivative. Using Density Functional Theory (DFT), we investigate how the molecular geometry and strength of the central acceptor unit impact the redox and spectroscopic properties. Notably, the incorporation of Pz and Tz moieties induces a significant redshift in the absorption and emission spectra, which extend into the near-infrared (NIR) region, simultaneously reducing their energy gaps (~1.4-1.6 eV). This shift is attributed to the increased coplanarity of the oligomeric inner core, both in the ground (S0) and excited (S1) states, due to the enhanced quinoidal character as supported by bond-length alternation (BLA) analysis. These structural changes promote better π-electron delocalization and facilitate photoinduced charge transfer processes in optoelectronic devices. Notably, we show that Pz- and Tz-containing molecules exhibit NIR electrochromic behavior and present ambivalent character in bulk heterojunction (BHJ) solar cells. Finally, theoretical calculations suggest that these molecules could serve as effective two-photon absorption (2PA) probes, further expanding their potential in optoelectronic applications.

Affiliations:
Zangoli M. - CNR-ISOF (IT)
Monti F. - CNR-ISOF (IT)
Zanelli A. - CNR-ISOF (IT)
Marinelli M. - other affiliation
Flammini S. - other affiliation
Spallacci N. - other affiliation
Zakrzewska A. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Salatelli E. - University of Bologna (IT)
Pierini F. - IPPT PAN
Di Maria F. - CNR-ISOF (IT)
9.  Zakrzewska A., Zargarian S.S., Rinoldi C., Gradys A.D., Jarząbek D.M., Zanoni M., Gualandi C., Lanzi M., Pierini F., Electrospun Poly(vinyl alcohol)-Based Conductive Semi-interpenetrating Polymer Network Fibrous Hydrogel: A Toolbox for Optimal Cross-Linking, ACS Materials Au, ISSN: 2694-2461, DOI: 10.1021/acsmaterialsau.3c00025, Vol.3, No.5, pp.464-482, 2023

Abstract:
Cross-linking of poly(vinyl alcohol) (PVA) creates a three-dimensional network by bonding adjacent polymer chains. The cross-linked structure, upon immersion in water, turns into a hydrogel, which exhibits unique absorption properties due to the presence of hydrophilic groups within the PVA polymer chains and, simultaneously, ceases to be soluble in water. The properties of PVA can be adjusted by chemical modification or blending with other substances, such as polymers, e.g., conductive poly[3-(potassium-5-butanoate)thiophene-2,5-diyl] (P3KBT). In this work, PVA-based conductive semi-interpenetrating polymer networks (semi-IPNs) are successfully fabricated. The systems are obtained as a result of electrospinning of PVA/P3KBT precursor solutions with different polymer concentrations and then cross-linking using “green”, environmentally safe methods. One approach consists of thermal treatment (H), while the second approach combines stabilization with ethanol and heating (E). The comprehensive characterization allows to evaluate the correlation between the cross-linking methods and properties of nanofibrous hydrogels. While both methods are successful, the cross-linking density is higher in the thermally cross-linked samples, resulting in lower conductivity and swelling ratio compared to the E-treated samples. Moreover, the H-cross-linked systems have better mechanical properties─lower stiffness and greater tensile strength. All the tested systems are biocompatible, and interestingly, due to the presence of P3KBT, they show photoresponsivity to solar radiation generated by the simulator. The results indicate that both methods of PVA cross-linking are highly effective and can be applied to a specific system depending on the target, e.g., biomedical or electronic applications.

Keywords:
poly(vinyl alcohol),poly[3-(potassium-5-butanoate)thiophene-2.5-diyl],electrospun nanofibers,cross-linking,fibrous hydrogel,semi-IPN

Affiliations:
Zakrzewska A. - IPPT PAN
Zargarian S.S. - IPPT PAN
Rinoldi C. - IPPT PAN
Gradys A.D. - IPPT PAN
Jarząbek D.M. - IPPT PAN
Zanoni M. - other affiliation
Gualandi C. - University of Bologna (IT)
Lanzi M. - University of Bologna (IT)
Pierini F. - IPPT PAN
10.  Zakrzewska A., Haghighat Bayan M.A., Nakielski P., Petronella F., De Sio L., Pierini F., Nanotechnology Transition Roadmap toward Multifunctional Stimuli-Responsive Face Masks, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.2c10335, Vol.14, No.41, pp.46123-46144, 2022

Abstract:
In recent times, the use of personal protective equipment, such as face masks or respirators, is becoming more and more critically important because of common pollution; furthermore, face masks have become a necessary element in the global fight against the COVID-19 pandemic. For this reason, the main mission of scientists has become the development of face masks with exceptional properties that will enhance their performance. The versatility of electrospun polymer nanofibers has determined their suitability as a material for constructing “smart” filter media. This paper provides an overview of the research carried out on nanofibrous filters obtained by electrospinning. The progressive development of the next generation of face masks whose unique properties can be activated in response to a specific external stimulus is highlighted. Thanks to additional components incorporated into the fiber structure, filters can, for example, acquire antibacterial or antiviral properties, self-sterilize the structure, and store the energy generated by users. Despite the discovery of several fascinating possibilities, some of them remain unexplored. Stimuli-responsive filters have the potential to become products of large-scale availability and great importance to society as a whole.

Keywords:
nanostructured face masks, stimuli-responsive nanomaterials, electrospun nanofibers, active filtration, smart filters, COVID-19, antipathogen

Affiliations:
Zakrzewska A. - IPPT PAN
Haghighat Bayan M.A. - IPPT PAN
Nakielski P. - IPPT PAN
Petronella F. - other affiliation
De Sio L. - Sapienza University of Rome (IT)
Pierini F. - IPPT PAN
11.  Ziai Y., Petronella F., Rinoldi C., Nakielski P., Zakrzewska A., Kowalewski T.A., Augustyniak W., Li X., Calogero A., Sabała I., Ding B., De Sio L., Pierini F., Chameleon-inspired multifunctional plasmonic nanoplatforms for biosensing applications, NPG Asia Materials, ISSN: 1884-4049, DOI: 10.1038/s41427-022-00365-9, Vol.14, pp.18-1-17, 2022

Abstract:
One of the most fascinating areas in the field of smart biopolymers is biomolecule sensing. Accordingly, multifunctional biomimetic, biocompatible, and stimuli-responsive materials based on hydrogels have attracted much interest. Within this framework, the design of nanostructured materials that do not require any external energy source is beneficial for developing a platform for sensing glucose in body fluids. In this article, we report the realization and application of an innovative platform consisting of two outer layers of a nanocomposite plasmonic hydrogel plus one inner layer of electrospun mat fabricated by electrospinning, where the outer layers exploit photoinitiated free radical polymerization, obtaining a compact and stable device. Inspired by the exceptional features of chameleon skin, plasmonic silver nanocubes are embedded into a poly(N-isopropylacrylamide)-based hydrogel network to obtain enhanced thermoresponsive and antibacterial properties. The introduction of an electrospun mat creates a compatible environment for the homogeneous hydrogel coating while imparting excellent mechanical and structural properties to the final system. Chemical, morphological, and optical characterizations were performed to investigate the structure of the layers and the multifunctional platform. The synergetic effect of the nanostructured system’s photothermal responsivity and antibacterial properties was evaluated. The sensing features associated with the optical properties of silver nanocubes revealed that the proposed multifunctional system is a promising candidate for glucose-sensing applications.

Affiliations:
Ziai Y. - IPPT PAN
Petronella F. - other affiliation
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Zakrzewska A. - IPPT PAN
Kowalewski T.A. - IPPT PAN
Augustyniak W. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Li X. - Donghua University (CN)
Calogero A. - Sapienza University of Rome (IT)
Sabała I. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Ding B. - Donghua University (CN)
De Sio L. - Sapienza University of Rome (IT)
Pierini F. - IPPT PAN

Category A Plus

IPPT PAN

logo ippt            Pawińskiego 5B, 02-106 Warsaw
  +48 22 826 12 81 (central)
  +48 22 826 98 15
 

Find Us

mapka
© Institute of Fundamental Technological Research Polish Academy of Sciences 2024