Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

Pracownicy

dr inż. Alicja Kosik-Kozioł

Zakład Biosystemów i Miękkiej Materii (ZBiMM)
Pracownia Zastosowań Nanomateriałów Polimerowych (PZNP)
stanowisko: adiunkt
telefon: (+48) 22 826 12 81 wewn.: 330
pokój: 320
e-mail:
ORCID: 0000-0002-2607-7271

Doktorat
2020-09-15 Three-dimensional tissue scaffolds fabrication for Osteochondral Tissue Engineering  (Politechnika Warszawska)
promotor -- dr hab. inż. Wojciech Święszkowski, PW
 

Ostatnie publikacje
1.  Nakielski P., Kosik-Kozioł A., Rinoldi C., Rybak D., Namdev M., Jacob W., Lehmann T., Głowacki M., Bogusz S., Rzepna M., Marinelli M., Lanzi M., Dror S., Sarah M., Dmitriy S., Pierini F., Injectable PLGA Microscaffolds with Laser-Induced Enhanced Microporosity for Nucleus Pulposus Cell Delivery, Small, ISSN: 1613-6810, DOI: 10.1002/smll.202404963, pp.2404963-1-15, 2024

Streszczenie:
Intervertebral disc (IVD) degeneration is a leading cause of lower back pain (LBP). Current treatments primarily address symptoms without halting the degenerative process. Cell transplantation offers a promising approach for early-stage IVD degeneration, but challenges such as cell viability, retention, and harsh host environments limit its efficacy. This study aimed to compare the injectability and biocompatibility of human nucleus pulposus cells (hNPC) attached to two types of microscaffolds designed for minimally invasive delivery to IVD. Microscaffolds are developed from poly(lactic-co-glycolic acid) (PLGA) using electrospinning and femtosecond laser structuration. These microscaffolds are tested for their physical properties, injectability, and biocompatibility. This study evaluates cell adhesion, proliferation, and survival in vitro and ex vivo within a hydrogel-based nucleus pulposus model. The microscaffolds demonstrate enhanced surface architecture, facilitating cell adhesion and proliferation. Laser structuration improved porosity, supporting cell attachment and extracellular matrix deposition. Injectability tests show that microscaffolds can be delivered through small-gauge needles with minimal force, maintaining high cell viability. The findings suggest that laser-structured PLGA microscaffolds are viable for minimally invasive cell delivery. These microscaffolds enhance cell viability and retention, offering potential improvements in the therapeutic efficiency of cell-based treatments for discogenic LBP.

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Rinoldi C. - IPPT PAN
Rybak D. - IPPT PAN
Namdev M. - inna afiliacja
Jacob W. - inna afiliacja
Lehmann T. - inna afiliacja
Głowacki M. - Jagiellonian University (PL)
Bogusz S. - inna afiliacja
Rzepna M. - inna afiliacja
Marinelli M. - inna afiliacja
Lanzi M. - University of Bologna (IT)
Dror S. - inna afiliacja
Sarah M. - inna afiliacja
Dmitriy S. - inna afiliacja
Pierini F. - IPPT PAN
200p.
2.  Kosik-Kozioł A., Nakielski P., Rybak D., Frączek W., Rinoldi C., Lanzi M., Grodzik M., Pierini F., Adhesive Antibacterial Moisturizing Nanostructured Skin Patch for Sustainable Development of Atopic Dermatitis Treatment in Humans, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.4c06662, Vol.16, No.25, pp.32128-32146, 2024

Streszczenie:
Atopic dermatitis (AD) is a chronic inflammatory skin disease with a complex etiology that lacks effective treatment. The therapeutic goals include alleviating symptoms, such as moisturizing and applying antibacterial and anti-inflammatory medications. Hence, there is an urgent need to develop a patch that effectively alleviates most of the AD symptoms. In this study, we employed a “green” cross-linking approach of poly(vinyl alcohol) (PVA) using glycerol, and we combined it with polyacrylonitrile (PAN) to fabricate core–shell (CS) nanofibers through electrospinning. Our designed structure offers multiple benefits as the core ensures controlled drug release and increases the strength of the patch, while the shell provides skin moisturization and exudate absorption. The efficient PVA cross-linking method facilitates the inclusion of sensitive molecules such as fermented oils. In vitro studies demonstrate the patches’ exceptional biocompatibility and efficacy in minimizing cell ingrowth into the CS structure containing argan oil, a property highly desirable for easy removal of the patch. Histological examinations conducted on an ex vivo model showed the nonirritant properties of developed patches. Furthermore, the eradication of Staphylococcus aureus bacteria confirms the potential use of CS nanofibers loaded with argan oil or norfloxacin, separately, as an antibacterial patch for infected AD wounds. In vivo patch application studies on patients, including one with AD, demonstrated ideal patches’ moisturizing effect. This innovative approach shows significant promise in enhancing life quality for AD sufferers by improving skin hydration and avoiding infections.

Słowa kluczowe:
atopic dermatitis, core−shell electrospun nanofibers, antibacterial, mucoadhesive, moisturizing patch

Afiliacje autorów:
Kosik-Kozioł A. - IPPT PAN
Nakielski P. - IPPT PAN
Rybak D. - IPPT PAN
Frączek W. - inna afiliacja
Rinoldi C. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Grodzik M. - inna afiliacja
Pierini F. - IPPT PAN
200p.
3.  Bartolewska M., Kosik-Kozioł A., Korwek Z., Krysiak Z., Devis M., Mazur M., Giuseppe F., Pierini F., Eumelanin-Enhanced Photothermal Disinfection of Contact Lenses Using a Sustainable Marine Nanoplatform Engineered with Electrospun Nanofibers, ADVANCED HEALTHCARE MATERIALS, ISSN: 2192-2659, DOI: 10.1002/adhm.202402431, pp.2402431-1-21, 2024

Streszczenie:
Bacterial keratitis (BK) is a severe eye infection commonly associated with Staphylococcus aureus (S. aureus), posing a significant risk to vision, especially among contact lens wearers. This research introduces a novel smart nanoplatform (deMS@cNF), developed from demineralized mussel shells (deMS) and reinforced with chitin (CT) nanofibrils, specifically designed for portable photothermal disinfection of contact lenses. The nanoplatform leverages the photothermal properties of eumelanin in mussel shells (MS), which, when activated by a simple bike flashlight, rapidly heats to temperatures up to 95 °C, effectively destroying bacterial contamination. In vitro tests demonstrate that the nanoplatform is biocompatible and non-toxic, making it suitable for medical applications. This study highlights an innovative approach to converting marine biowaste into a safe, effective, and low-cost portable method for disinfecting contact lenses, showcasing the potential of the deMS@cNF platform for broader antimicrobial applications.

Afiliacje autorów:
Bartolewska M. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Korwek Z. - IPPT PAN
Krysiak Z. - IPPT PAN
Devis M. - inna afiliacja
Mazur M. - inna afiliacja
Giuseppe F. - inna afiliacja
Pierini F. - IPPT PAN
140p.
4.  Haghighat Bayan M.A., Rinoldi C., Kosik-Kozioł A., Bartolewska M., Rybak D., Zargarian S., Shah S., Krysiak Z., Zhang S., Lanzi M., Nakielski P., Ding B., Pierini F., Solar-to-NIR Light Activable PHBV/ICG Nanofiber-Based Face Masks with On-Demand Combined Photothermal and Photodynamic Antibacterial Properties, Advanced Materials Technologies, ISSN: 2365-709X, DOI: 10.1002/admt.202400450, pp.2400450-1-18, 2024

Streszczenie:
Hierarchical nanostructures fabricate by electrospinning in combination with light-responsive agents offer promising scenarios for developing novel activable antibacterial interfaces. This study introduces an innovative antibacterial face mask developed from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers integrated with indocyanine green (ICG), targeting the urgent need for effective antimicrobial protection for community health workers. The research focuses on fabricating and characterizing this nanofibrous material, evaluating the mask's mechanical and chemical properties, investigating its particle filtration, and assessing antibacterial efficacy under photothermal conditions for reactive oxygen species (ROS) generation. The PHBV/ICG nanofibers are produced using an electrospinning process, and the nanofibrous construct's morphology, structure, and photothermal response are investigated. The antibacterial efficacy of the nanofibers is tested, and substantial bacterial inactivation under both near-infrared (NIR) and solar irradiation is demonstrated due to the photothermal response of the nanofibers. The material's photothermal response is further analyzed under cyclic irradiation to simulate real-world conditions, confirming its durability and consistency. This study highlights the synergistic impact of PHBV and ICG in enhancing antibacterial activity, presenting a biocompatible and environmentally friendly solution. These findings offer a promising path for developing innovative face masks that contribute significantly to the field of antibacterial materials and solve critical public health challenges.

Afiliacje autorów:
Haghighat Bayan M.A. - IPPT PAN
Rinoldi C. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Bartolewska M. - IPPT PAN
Rybak D. - IPPT PAN
Zargarian S. - IPPT PAN
Shah S. - IPPT PAN
Krysiak Z. - IPPT PAN
Zhang S. - inna afiliacja
Lanzi M. - University of Bologna (IT)
Nakielski P. - IPPT PAN
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
100p.
5.  Zargarian S., Zakrzewska A., Kosik-Kozioł A., Bartolewska M., Shah S., Li X., Su Q., Petronella F., Marinelli M., De Sio L., Lanzi M., Ding B., Pierini F., Advancing resource sustainability with green photothermal materials: Insights from organic waste-derived and bioderived sources, nanotechnology reviews, ISSN: 2191-9097, DOI: 10.1515/ntrev-2024-0100, Vol.13, No.1, pp.20240100-1-39, 2024

Streszczenie:
Recently, there has been a surge of interest in developing new types of photothermal materials driven by the ongoing demand for efficient energy conversion, environmental concerns, and the need for sustainable solutions. However, many existing photothermal materials face limitations such as high production costs or narrow absorption bands, hindering their widespread application. In response to these challenges, researchers have redirected their focus toward harnessing the untapped potential of organic waste-derived and bioderived materials. These materials, with photothermal properties derived from their intrinsic composition or transformative processes, offer a sustainable and cost-effective alternative. This review provides an extended categorization of organic waste-derived and bioderived materials based on their origin. Additionally, we investigate the mechanisms underlying the photothermal properties of these materials. Key findings highlight their high photothermal efficiency and versatility in applications such as water and energy harvesting, desalination, biomedical applications, deicing, waste treatment, and environmental remediation. Through their versatile utilization, they demonstrate immense potential in fostering sustainability and support the transition toward a greener and more resilient future. The authors’ perspective on the challenges and potentials of platforms based on these materials is also included, highlighting their immense potential for real-world implementation.

Słowa kluczowe:
photothermal materials, organic waste valorization, bioderived materials

Afiliacje autorów:
Zargarian S. - IPPT PAN
Zakrzewska A. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Bartolewska M. - IPPT PAN
Shah S. - IPPT PAN
Li X. - Donghua University (CN)
Su Q. - inna afiliacja
Petronella F. - inna afiliacja
Marinelli M. - inna afiliacja
De Sio L. - inna afiliacja
Lanzi M. - University of Bologna (IT)
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
70p.
6.  Zargarian S., Kupikowska-Stobba B., Kosik-Kozioł A., Bartolewska M., Zakrzewska A., Rybak D., Bochenek K., Osial M., Pierini F., Light-responsive biowaste-derived and bio-inspired textiles: Dancing between bio-friendliness and antibacterial functionality, Materials Today Chemistry, ISSN: 2468-5194, DOI: 10.1016/j.mtchem.2024.102281, Vol.41, pp.102281-1-15, 2024

Streszczenie:
Functional antibacterial textiles fabricated from a hybrid of organic waste-derived and bio-inspired materials offer sustainable solutions for preventing microbial infections. In this work, we developed a novel antibacterial textile created through the valorization of spent coffee grounds (SCG). Electrospinning and electrospraying techniques were employed to integrate the biowaste within a polymeric nanofiber matrix, ensuring uniform particle distribution and providing structural support for enhanced applicability. Modification with polydopamine (PDA) significantly enhanced the textile's photothermal performance. Specific attention was paid to understanding the relation between temperature change and key variables, including the surrounding liquid volume, textile layer stacking, and applied laser power. Developed platforms demonstrated excellent photothermal stability. While the SCG-based textile demonstrated exceptional biocompatibility, the PDA-modified textile effectively eradicated Staphylococcus aureus (S. aureus) under near-infrared (NIR) irradiation. The developed textiles in our work demonstrate a dynamic balance between biocompatibility and on-demand antibacterial functionality, offering adaptable solutions in accordance with the desired application.

Słowa kluczowe:
Organic waste valorization, Spent coffee grounds, Micro-nanostructured textiles, Bio-inspired photothermal agents, Polydopamine, Antibacterial textiles

Afiliacje autorów:
Zargarian S. - IPPT PAN
Kupikowska-Stobba B. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Bartolewska M. - IPPT PAN
Zakrzewska A. - IPPT PAN
Rybak D. - IPPT PAN
Bochenek K. - IPPT PAN
Osial M. - IPPT PAN
Pierini F. - IPPT PAN
70p.
7.  Ziai Y., Lanzi M., Rinoldi C., Zargarian S. S., Zakrzewska A., Kosik-Kozioł A., Nakielski P., Pierini F., Developing strategies to optimize the anchorage between electrospun nanofibers and hydrogels for multi-layered plasmonic biomaterials, Nanoscale Advances, ISSN: 2516-0230, DOI: 10.1039/d3na01022h, pp.1-13, 2024

Streszczenie:
Polycaprolactone (PCL), a recognized biopolymer, has emerged as a prominent choice for diverse biomedical endeavors due to its good mechanical properties, exceptional biocompatibility, and tunable properties. These attributes render PCL a suitable alternative biomaterial to use in biofabrication, especially the electrospinning technique, facilitating the production of nanofibers with varied dimensions and functionalities. However, the inherent hydrophobicity of PCL nanofibers can pose limitations. Conversely, acrylamide-based hydrogels, characterized by their interconnected porosity, significant water retention, and responsive behavior, present an ideal matrix for numerous biomedical applications. By merging these two materials, one can harness their collective strengths while potentially mitigating individual limitations. A robust interface and effective anchorage during the composite fabrication are pivotal for the optimal performance of the nanoplatforms. Nanoplatforms are subject to varying degrees of tension and physical alterations depending on their specific applications. This is particularly pertinent in the case of layered nanostructures, which require careful consideration to maintain structural stability and functional integrity in their intended applications. In this study, we delve into the influence of the fiber dimensions, orientation and surface modifications of the nanofibrous layer and the hydrogel layer's crosslinking density on their intralayer interface to determine the optimal approach. Comprehensive mechanical pull-out tests offer insights into the interfacial adhesion and anchorage between the layers. Notably, plasma treatment of the hydrophobic nanofibers and the stiffness of the hydrogel layer significantly enhance the mechanical effort required for fiber extraction from the hydrogels, indicating improved anchorage. Furthermore, biocompatibility assessments confirm the potential biomedical applications of the proposed nanoplatforms.

Afiliacje autorów:
Ziai Y. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Rinoldi C. - IPPT PAN
Zargarian S. S. - IPPT PAN
Zakrzewska A. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Nakielski P. - IPPT PAN
Pierini F. - IPPT PAN
20p.
8.  Nakielski P., Rybak D., Jezierska-Woźniak K., Rinoldi C., Sinderewicz E., Staszkiewicz-Chodor J., Haghighat Bayan M.A., Czelejewska W., Urbanek-Świderska O., Kosik-Kozioł A., Barczewska M., Skomorowski M., Holak P., Lipiński S., Maksymowicz W., Pierini F., Minimally invasive intradiscal delivery of BM-MSCs via fibrous microscaffold carriers, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.3c11710, pp.1-16, 2023

Streszczenie:
Current treatments of degenerated intervertebral discs often provide only temporary relief or address specific causes, necessitating the exploration of alternative therapies. Cell-based regenerative approaches showed promise in many clinical trials, but
limitations such as cell death during injection and a harsh disk environment hinder their effectiveness. Injectable microscaffolds offer a solution by providing a supportive microenvironment for cell delivery and enhancing bioactivity. This study evaluated the
safety and feasibility of electrospun nanofibrous microscaffolds modified with chitosan (CH) and chondroitin sulfate (CS) for treating degenerated NP tissue in a large animal model. The microscaffolds facilitated cell attachment and acted as an effective delivery system, preventing cell leakage under a high disc pressure. Combining microscaffolds with bone marrow-derived mesenchymal stromal cells demonstrated no cytotoxic effects and proliferation over the entire microscaffolds. The administration of cells attached to microscaffolds into the NP positively influenced the regeneration process of the intervertebral disc. Injectable poly(L-lactide-co-glycolide) and poly(L-lactide) microscaffolds enriched with CH or CS, having a fibrous structure, showed the potential to promote intervertebral disc regeneration. These features collectively address critical challenges in the fields of tissue engineering and regenerative medicine, particularly in the context of intervertebral disc degeneration.

Słowa kluczowe:
microscaffolds,cell carriers,injectable biomaterials,intervertebral disc,laser micromachining,electrospinning

Afiliacje autorów:
Nakielski P. - IPPT PAN
Rybak D. - IPPT PAN
Jezierska-Woźniak K. - inna afiliacja
Rinoldi C. - IPPT PAN
Sinderewicz E. - inna afiliacja
Staszkiewicz-Chodor J. - inna afiliacja
Haghighat Bayan M.A. - IPPT PAN
Czelejewska W. - inna afiliacja
Urbanek-Świderska O. - IPPT PAN
Kosik-Kozioł A. - IPPT PAN
Barczewska M. - University of Warmia and Mazury in Olsztyn (PL)
Skomorowski M. - inna afiliacja
Holak P. - inna afiliacja
Lipiński S. - inna afiliacja
Maksymowicz W. - University of Warmia and Mazury in Olsztyn (PL)
Pierini F. - IPPT PAN
200p.
9.  Kosik-Kozioł A., Heljak M., Święszkowski W., Mechanical properties of hybrid triphasic scaffolds for osteochondral tissue engineering, Materials Letters, ISSN: 0167-577X, DOI: 10.1016/j.matlet.2019.126893, Vol.261, pp.126893-1-5, 2020

Streszczenie:
Reproducing the advanced complexity of native tissue by means of the 3D multi-functional construct is a promising tissue engineering approach to osteochondral tissue regeneration. In this study, we present a porous 3D construct composed of three zones responsible for the regeneration of non-calcified cartilage, calcified cartilage and subchondral bone. These three zones of the hybrid were composed of modified biopolymers: (i) alginate (Alg) reinforced by short polylactide (PLA) fibres, (ii) alginate and gelatine methacrylate (GelMA) combined with ß-tricalcium phosphate particles (TCP), (iii) 3D printed polycaprolactone scaffold subsequently modified with the use of an innovative solvent treatment method based on acetone and ultrasound stimulation, respectively. Combining the advanced deposition systems based on: (i) 3D printing coupled with a spray crosslinking system, (ii) an innovative deposition system based on a coaxial-needle extruder, (iii) fused deposition modelling (FDM) connected with post-fabrication treatment, allows us to fabricate the triphasic construct that emulates the structure and properties of the native osteochondral tissue. The aim of the study was to investigate the mechanical properties of the fabricated hybrid and its individual zones. Our results demonstrate the load-bearing capabilities of TC, but nevertheless it should be implanted below the surface line of host cartilage to protect it from strong stresses, at the same time allowing native host tissues to grow into it.

Słowa kluczowe:
Triphasic scaffold, Osteochondral tissue engineering, Mechanical properties, Hydrogel with nanofillers, Modified PCL

Afiliacje autorów:
Kosik-Kozioł A. - inna afiliacja
Heljak M. - Politechnika Warszawska (PL)
Święszkowski W. - inna afiliacja
10.  Kosik-Kozioł A., Graham E., Jaroszewicz J., Chlanda A., Kumar P.S., IvanovskI S., Święszkowski W., Vaquette C., Surface Modification of 3D Printed Polycaprolactone Constructs via a Solvent Treatment: Impact on Physical and Osteogenic Properties, ACS BIOMATERIALS SCIENCE & ENGINEERING, ISSN: 2373-9878, DOI: 10.1021/acsbiomaterials.8b01018, Vol.5, No.1, pp.318-328, 2019

Streszczenie:
One promising strategy to reconstruct bone defects relies on 3D printed porous structures. In spite of several studies having been carried out to fabricate controlled, interconnected porous constructs, the control over surface features at, or below, the microscopic scale remains elusive for 3D polymeric scaffolds. In this study, we developed and refined a methodology which can be applied to homogeneously and reproducibly modify the surface of polymeric 3D printed scaffolds. We have demonstrated that the combination of a polymer solvent and the utilization of ultrasound was essential for achieving appropriate surface modification without damaging the structural integrity of the construct. The modification created on the scaffold profoundly affected the macroscopic and microscopic properties of the scaffold with an increased roughness, greater surface area, and reduced hydrophobicity. Furthermore, to assess the performance of such materials in bone tissue engineering, human mesenchymal stem cells (hMSC) were cultured in vitro on the scaffolds for up to 7 days. Our results demonstrate a stronger commitment toward early osteogenic differentiation of hMSC. Finally, we demonstrated that the increased in the specific surface area of the scaffold did not necessarily correlate with improved adsorption of protein and that other factors, such as surface chemistry and hydrophilicity, may also play a major role.

Słowa kluczowe:
surface modification, solvent treatment, polycaprolactone, BMP-2 adsorption

Afiliacje autorów:
Kosik-Kozioł A. - inna afiliacja
Graham E. - inna afiliacja
Jaroszewicz J. - inna afiliacja
Chlanda A. - Politechnika Warszawska (PL)
Kumar P.S. - inna afiliacja
IvanovskI S. - inna afiliacja
Święszkowski W. - inna afiliacja
Vaquette C. - inna afiliacja
140p.
11.  Kosik-Kozioł A., Costantini M., Mróz A., Idaszek J., Heljak M., Jaroszewicz J., Kijeńska E., Szöke K., Frerker N., Barbetta A., Brinchmann J.E., Święszkowski W., 3D bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering, Biofabrication, ISSN: 1758-5082, DOI: 10.1088/1758-5090/ab15cb, Vol.11, No.3, pp.035016-1-29, 2019

Streszczenie:
One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while—despite its key role in the osteochondral region—only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry.

Słowa kluczowe:
alginate, gelatin methacrylate, ß-tricalcium phosphate TCP, bioprinting, coaxial needle, calcified cartilage

Afiliacje autorów:
Kosik-Kozioł A. - inna afiliacja
Costantini M. - Sapienza University of Rome (IT)
Mróz A. - inna afiliacja
Idaszek J. - inna afiliacja
Heljak M. - Politechnika Warszawska (PL)
Jaroszewicz J. - inna afiliacja
Kijeńska E. - inna afiliacja
Szöke K. - inna afiliacja
Frerker N. - inna afiliacja
Barbetta A. - Sapienza University of Rome (IT)
Brinchmann J.E. - inna afiliacja
Święszkowski W. - inna afiliacja
140p.
12.  Heljak M.K., Moczulska-Heljak M., Choińska E., Chlanda A., Kosik-Kozioł A., Jaroszewicz T., Jaroszewicz J., Święszkowski W., Micro and nanoscale characterization of poly(DL-lactic-co-glycolic acid) films subjected to the L929 cells and the cyclic mechanical load, Micron, ISSN: 0968-4328, DOI: 10.1016/j.micron.2018.09.004, Vol.115, pp.64-72, 2018

Streszczenie:
In this paper, the effect of the presence of L929 fibroblast cells and a cyclic load application on the kinetics of the degradation of amorphous PLGA films was examined. Complex micro and nano morphological, mechanical and physico-chemical studies were performed to assess the degradation of the tested material. For this purpose, molecular weight, glass transition temperature, specimen morphology (SEM, μCT) and topography (AFM) as well as the stiffness of the material were measured. The study showed that the presence of living cells along with a mechanical load accelerates the PLGA degradation in comparison to the degradation occurring in acellular media: PBS and DMEM. The drop in molecular weight observed was accompanied by a distinct increase in the tensile modulus and surface roughness, especially in the case of the film degradation in the presence of cells. The suspected cause of the rise in stiffness during the degradation of PLGA films is a reduction in the molecular mobility of the distinctive superficial layer resulting from severe structural changes caused by the surface degradation. In conclusion, all the micro and nanoscale properties of amorphous PLGA considered in the study are sensitive to the presence of L929 cells, as well as to a cyclic load applied during the degradation process.

Słowa kluczowe:
L929, aliphatic polyester, stiffness rise

Afiliacje autorów:
Heljak M.K. - Politechnika Warszawska (PL)
Moczulska-Heljak M. - inna afiliacja
Choińska E. - Politechnika Warszawska (PL)
Chlanda A. - Politechnika Warszawska (PL)
Kosik-Kozioł A. - inna afiliacja
Jaroszewicz T. - Politechnika Warszawska (PL)
Jaroszewicz J. - inna afiliacja
Święszkowski W. - inna afiliacja
30p.
13.  Kosik-Kozioł A., Costantini M., Bolek T., Szöke K., Barbetta A., Brinchmann J., Święszkowski W., PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration, Biofabrication, ISSN: 1758-5082, DOI: 10.1088/1758-5090/aa90d7, Vol.9, No.4, pp.044105-1-13, 2017

Streszczenie:
In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days. Live/dead assays at day 0, 3, 7 and 14 of in vitro culture showed that human chondrocytes were retained and highly viable (∼80%) within the 3D deposited hydrogel filaments, thus confirming that the fabricated composites materials represent a valid solution for tissue engineering applications. Finally, we show that the embedded chondrocytes during all the in vitro culture maintain a round morphology, a key parameter for a proper deposition of neocartilage extracellular matrix.

Słowa kluczowe:
alginate, PLA, short fibers, hydrogel reinforcement, chondrocytes

Afiliacje autorów:
Kosik-Kozioł A. - inna afiliacja
Costantini M. - Sapienza University of Rome (IT)
Bolek T. - inna afiliacja
Szöke K. - inna afiliacja
Barbetta A. - Sapienza University of Rome (IT)
Brinchmann J. - inna afiliacja
Święszkowski W. - inna afiliacja
14.  Kosik-Kozioł A, Luchowska U., Święszkowski W., Electrolyte alginate/poly-l-lysine membranes for connective tissue development, Materials Letters, ISSN: 0167-577X, DOI: 10.1016/j.matlet.2016.08.032, Vol.184, No.1, pp.104-107, 2016

Streszczenie:
The aim of this study was to change the surface of sodium alginate hydrogel by electrostatic binding of poly-l-lysine (PLL) in order to provide more advantageous conditions for connective tissue development. Its impact on L929 mouse fibroblast adhesion, morphology and viability was investigated. Analysis of the material microstructure has shown that performed modification increased surface roughness. It also altered the swelling properties of alginate hydrogel, resulting in less rapid water absorption. Mouse fibroblasts seeded on regular alginate and alginate modified with PLL exhibited different behaviour. The presence of PLL turned out to promote cell adhesion and F-actin spreading, resulting in significantly increased number of viable cells 96 h after seeding.

Słowa kluczowe:
Alginate, Poly-l-lysine, Electrolyte membranes, Cell adhesion

Afiliacje autorów:
Kosik-Kozioł A - inna afiliacja
Luchowska U. - inna afiliacja
Święszkowski W. - inna afiliacja
15.  Wszola M., Idaszek J., Berman A., Kosik-Kozioł A, Gorski L., Jozwik A., Dobrzyn A., Cudnoch-Jędrzejewska A., Kaminski A., Wrzesien R., Serwanska-Swietek M., Chmura A., Kwiatkowski A., Święszkowski W., Bionic Pancreas and Bionic Organs – how far we are from the success, Medtube Science, ISSN: 2353-5695, Vol.3, No.3, pp.25-27, 2015

Streszczenie:
The progress in the treatment of chronic diseases of civilization that occurred in recent years, led to a significant prolongation of median survival time of the developed countries societies. Organ transplantation has revolutionized medicine as it became possible to replace an irreversibly diseased organ. However, at the moment we can observe a significant shortage of organs for transplantation, which forces doctors to accept those coming from more and more expanding criteria donors. No doubt, the number of donors, at best, will certainly not grow. Tissue engineering and regenerative medicine methods are extremely promising, in particular bioprinting of tissues and organs, which begun to develop at the beginning of the XXI century. Article highlights possible future direction of organ transplantation.

Afiliacje autorów:
Wszola M. - inna afiliacja
Idaszek J. - inna afiliacja
Berman A. - inna afiliacja
Kosik-Kozioł A - inna afiliacja
Gorski L. - inna afiliacja
Jozwik A. - inna afiliacja
Dobrzyn A. - inna afiliacja
Cudnoch-Jędrzejewska A. - inna afiliacja
Kaminski A. - inna afiliacja
Wrzesien R. - inna afiliacja
Serwanska-Swietek M. - inna afiliacja
Chmura A. - inna afiliacja
Kwiatkowski A. - Military Institute of Medicine (PL)
Święszkowski W. - inna afiliacja

Abstrakty konferencyjne
1.  Kosik-Kozioł A., Rybak D., Rinoldi C., Nakielski P., Pierini F., Ferment Oil-Laden Core-Shell Electrospun Nanofibers for Wound Healing Application, Frontiers in Polymer Science 2023 — Seventh International Symposium Frontiers in Polymer Science, 2023-05-30/06-01, Gothenburg (SE), pp.P2.062-P2.062, 2023

Streszczenie:
Hard-to-heal wounds represent a significant public health problem that often carries a considerable risk of health complications with a negative impact on the quality of a patient's life [1]. The lack of effective treatments for skin damage can be attributed in part to the complexity of a physiological process occurring during the healing and microbial invasion from both resident and exogenous bacteria [2,3]. This research aimed to meet these challenges by developing a multifunctional core-shell nanofiber scaffold releasing the drugs and consisted antimicrobial peptides that hinder bacterial colonization while accelerating the healing process. Core-shell electrospun naofiber systems can control the biomolecule release profile providing sustainable drugs for wound healing. Implemented antimicrobial peptides effectively destroy a large spectrum of pathogens by contact with the cell membrane, decreasing the rate of antibiotic resistance in our healthcare system. The combination of the coaxial system with electrospinning allowed to obtain well-defined fibers. In this study, highly hydrophilic polyvinyl alcohol was confined into water-stable electrospun fibers using optimized polymer blends and cross-linking methods. All employed structures showed ideal morphology, construct's stability over time, and appropriate drug release profile as well as high-cell viability and antimicrobial properties. The developed multifunctional platforms represent a robust and valid candidate for fabricating skin dressings, accelerating the healing of patients' wounds while protecting against bacterial infection.

Słowa kluczowe:
electrospinning, PVA, Green crosslinking

Afiliacje autorów:
Kosik-Kozioł A. - IPPT PAN
Rybak D. - IPPT PAN
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Pierini F. - IPPT PAN

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